B型DNA寡聚物中不可交换质子的共振归属概述
Resonance assignments of non-exchangeable protons in B type DNA oligomers, an overview.
作者信息
van de Ven F J, Hilbers C W
机构信息
Department of Biophysical Chemistry, University of Nijmegen, The Netherlands.
出版信息
Nucleic Acids Res. 1988 Jul 11;16(13):5713-26. doi: 10.1093/nar/16.13.5713.
Abstract
The chemical shifts of 1H resonances of non exchangeable protons (except H5', H5" and adenine H2) of over six hundred nucleotides have been collected. The influence which the base of the nucleotide itself as well as the bases on its 5' and 3' side exert on the chemical shifts of the various resonances has been investigated. Most of the resonances appear to be predominantly influenced by only one base. For H2', H2", H3', H4' and H6/H8 this is the base of the central nucleotide, for H5(C) and CH3(T) it is the one on the 5' side and for H1' it is the one on the 3' side. Chemical shift distribution profiles are presented which allow an estimation of the probability of finding a particular resonance at a particular position in the spectrum.
摘要
已收集了六百多个核苷酸的非交换质子（H5'、H5"和腺嘌呤H2除外）的1H共振化学位移。研究了核苷酸自身的碱基以及其5'和3'侧的碱基对各种共振化学位移的影响。大多数共振似乎主要仅受一个碱基影响。对于H2'、H2"、H3'、H4'和H6/H8，是中央核苷酸的碱基；对于H5(C)和CH3(T)，是5'侧的碱基；对于H1'，是3'侧的碱基。给出了化学位移分布图谱，可据此估计在光谱特定位置找到特定共振的概率。
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