1 Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA.
2 Interdisciplinary Materials Science Program, Vanderbilt University, Nashville, TN, USA.
SLAS Technol. 2018 Dec;23(6):592-598. doi: 10.1177/2472630318775059. Epub 2018 May 22.
The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump systems. Currently, researchers in the field of tissue engineering require the use of pump systems that are in general large, expensive, and generically designed. Overall, these pumps often fail to meet the unique demands of perfusing clinically useful tissue constructs. Here, we describe a pumping platform that overcomes these limitations and enables scalable perfusion of large, three-dimensional hydrogels. We demonstrate the ability to perfuse multiple separate channels inside hydrogel slabs using a preprogrammed schedule that dictates pumping speed and time. The use of this pump system to perfuse channels in large-scale engineered tissue scaffolds sustained cell viability over several weeks.
几种泵系统的发展促进了需要持续、控制灌注的工程化血管化组织和器官的制造。目前，组织工程领域的研究人员需要使用通常体积大、价格昂贵且通用设计的泵系统。总的来说，这些泵往往无法满足灌注临床有用的组织构建体的独特需求。在这里，我们描述了一种克服这些限制并能够对大型三维水凝胶进行可扩展灌注的泵送平台。我们展示了使用预先编程的方案来控制泵送速度和时间，从而在水凝胶板内灌注多个单独通道的能力。使用该泵系统灌注大型工程化组织支架中的通道可使细胞活力维持数周。
