Perfileva A I, Tsivileva O M, Drevko Ya B, Ibragimova D N, Koftin O V
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, 164033, Russia.
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410049, Russia.
Dokl Biol Sci. 2018 Mar;479(1):67-69. doi: 10.1134/S0012496618020072. Epub 2018 May 22.
The impact of selenium biocomposites obtained from the medicinal macrobasidiomycetes Ganoderma lucidum, Grifola umbellata, Laetiporus sulphureus, Lentinula edodes, and Pleurotus ostreatus on the viability and biofilm formation capability of the phytopathogenic Gram-positive bacterium Clavibacter michiganensis ssp. sepedonicus (Spieck. et Kotth.) (Cms) was studied. Impairment of bacterial cell viability resulting from their incubation with biocomposites was shown. The decisive role of the composites' selenium component on the biological activity under question was established. The dependence of antimicrobial effect of the selenium-containing specimen on the mushroom systematic position was revealed. The maximal activity was found for the biocomposites based on the extracellular metabolites of L. edodes and G. lucidum. When the biopolymer specimen of fungal origin was added to bacterial suspension, the Cms capability of forming biofilms was found to be distinctly dependent of the biocomposite type, and it was substantially reduced in a number of cases.
研究了从药用大型担子菌灵芝、猪苓、硫黄多孔菌、香菇和平菇中获得的硒生物复合材料对植物病原革兰氏阳性细菌密歇根棒杆菌马铃薯环腐亚种（Spieck. et Kotth.）（Cms）的活力和生物膜形成能力的影响。结果表明，该生物复合材料与细菌共同孵育会导致细菌细胞活力受损。确定了复合材料中硒成分对上述生物活性的决定性作用。揭示了含硒样品的抗菌效果与蘑菇系统位置的相关性。发现基于香菇和灵芝细胞外代谢产物的生物复合材料具有最大活性。当将真菌来源的生物聚合物样品添加到细菌悬液中时，发现Cms形成生物膜的能力明显依赖于生物复合材料的类型，并且在许多情况下会大幅降低。
