AbouShabana N M, AbdelKader R, Abdel-Rahman S, Abdel-Gawad H S, Abdel-Galil A M
Aquaculture Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt.
Zoology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Fish Physiol Biochem. 2018 Aug;44(4):1241-1251. doi: 10.1007/s10695-018-0517-x. Epub 2018 May 22.
The current study was conducted to investigate the effect of ExcelMOS® in enhancing the immune system of Sparus aurata broodstock and their impact on offspring health through displaying the maternal transfer of immunity. Broodstock were divided into two groups: one was injected intraperitoneally with ExcelMOS® 1 month before spawning, while the other group was used as a control (without injection). Comprehensive increase in survival rate was observed for larvae hatched from ExcelMOS®-injected broodstock than those of the control (P ≤ 0.05). Hematological analysis showed increases in leukocyte count and hematocrit percentage (P ≤ 0.05) and significant enhancement in immune assays as phagocytic, respiratory burst, lysozyme activities in ExcelMOS®-injected broodstock (P ≤ 0.05). Additionally, total immunoglobulin levels in the serum, eggs, and larvae resulted from ExcelMOS®-injected broodstock were highly significant (P ≤ 0.05) than those in the control ones. Transmission electron microscopy and semi-thin sections in posterior intestine of ExcelMOS®-injected broodstock revealed reinforcement of the epithelial barrier structure, intestinal integrity, and functionality in combination with the stimulation of innate immune system. In conclusion, immunostimulation of Sparus aurata broodstock using ExcelMOS® has improved survival of larvae and enhanced both innate and adaptive immune defense mechanisms. Further investigations are required to show the effect of ExcelMOS® on fish cultured in intensive culture systems.
本研究旨在通过展示免疫的母源传递来探究ExcelMOS®对金头鲷亲鱼免疫系统的增强作用及其对后代健康的影响。亲鱼被分为两组：一组在产卵前1个月腹腔注射ExcelMOS®，另一组作为对照（未注射）。观察到从注射ExcelMOS®的亲鱼孵化出的幼虫的存活率全面提高，高于对照组（P≤0.05）。血液学分析显示，注射ExcelMOS®的亲鱼白细胞计数和血细胞比容百分比增加（P≤0.05），吞噬、呼吸爆发、溶菌酶活性等免疫检测显著增强（P≤0.05）。此外，注射ExcelMOS®的亲鱼产生的血清、卵和幼虫中的总免疫球蛋白水平比对照组高得多（P≤0.05）。对注射ExcelMOS®的亲鱼后肠进行的透射电子显微镜和半薄切片显示，上皮屏障结构、肠道完整性和功能得到加强，同时先天免疫系统受到刺激。总之，使用ExcelMOS®对金头鲷亲鱼进行免疫刺激提高了幼虫的存活率，并增强了先天和适应性免疫防御机制。需要进一步研究以表明ExcelMOS®对集约化养殖系统中养殖的鱼类的影响。
