从鳞状细胞癌中分离癌症干细胞。
Isolation of Cancer Stem Cells from Squamous Cell Carcinoma.
作者信息
Fontenete Silvia, Perez-Moreno Mirna
机构信息
Section for Cell Biology and Physiology, Department of Biology, Faculty of Science, University of Copenhagen, Copenhagen Ø, Denmark.
出版信息
Methods Mol Biol. 2019;1879:407-414. doi: 10.1007/7651_2018_162.
Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.
在包括鳞状细胞癌（SSC）在内的多种癌症类型中都能发现不同的癌症干细胞（CSC）群体。各种报告表明，CSC在不同类型癌症的复发中起着关键作用。CSC能够自我更新，并具有引发具有转移性癌症特征的继发性肿瘤的潜力，从而维持肿瘤生长。因此，开发分离CSC的方法是探索CSC维持机制的关键步骤。在本章中，我们提供了一种使用免疫荧光标记从皮肤SSC中分离CSC的方法，以便通过荧光激活细胞分选（FACS）对CSC进行特异性纯化。该方法基于使用CSC膜标记物，也能够从不同的小鼠品系中分离CSC。
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