Guevara Louise, Abdelgawad Azza, Onzere Cynthia, Greenwood Alex D, Davidson Zeke, Bishop Richard, Mutinda Mathew
1 Kolmarden Zoo, Veterinärkliniken, 618 92 Kolmården, Sweden.
2 Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315 Berlin, Germany.
J Wildl Dis. 2018 Oct;54(4):848-851. doi: 10.7589/2018-01-003. Epub 2018 May 24.
Equid herpesviruses types 1 (EHV-1) and 9 (EHV-9) are unusual among herpesviruses in that they lack strong host specificity, and the full extent of their host range remains unclear. The virus establishes latency for long periods and can be reactivated and shed, resulting in clinical disease in susceptible species. A sensitive and specific peptide-based enzyme-linked immunosorbent assay was developed to study the seroprevalence of both viruses in a broad range of species among both wild and captive populations. We used this assay to study the seroprevalences of EHV-1 and EHV-9 in a natural population of the highly endangered Grévy's zebra ( Equus grevyi) in Kenya, sampled during a 4-yr period (2012-15). The results were compared with those obtained from captive Grévy's zebras from a previous study. The wild population had a significantly higher seroprevalence of EHV-9 compared with the captive population, suggesting that captivity might reduce exposure to this serotype. In contrast, the seroprevalences of EHV-1 between captive and wild groups was not significantly different. The seroprevalence of EHV-9 was not significantly higher than EHV-1 in zebras within the wild Kenyan population.
1型马疱疹病毒（EHV-1）和9型马疱疹病毒（EHV-9）在疱疹病毒中较为特殊，因为它们缺乏很强的宿主特异性，其宿主范围的全貌仍不清楚。该病毒可长期潜伏，能够重新激活并排出体外，从而在易感物种中引发临床疾病。我们开发了一种灵敏且特异的基于肽的酶联免疫吸附测定法，以研究这两种病毒在野生和圈养种群中多种物种的血清流行率。我们利用该测定法研究了肯尼亚极度濒危的细纹斑马（Equus grevyi）自然种群中EHV-1和EHV-9的血清流行率，样本采集于4年期间（2012年至2015年）。将结果与之前一项研究中圈养细纹斑马的结果进行了比较。与圈养种群相比，野生种群中EHV-9的血清流行率显著更高，这表明圈养可能会减少对这种血清型的接触。相比之下，圈养组和野生组之间EHV-1的血清流行率没有显著差异。在肯尼亚野生斑马种群中，EHV-9的血清流行率并不显著高于EHV-1。
