Laboratory of Molecular Microbiology, Institute of Biomedical Sciences (ICBIM), Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil.
Clinical Wards, School of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil.
Microb Drug Resist. 2018 Dec;24(10):1441-1449. doi: 10.1089/mdr.2018.0043. Epub 2018 May 24.
Carbapenemase-producing organisms are pandemic and a significant threat to public health. We investigated the clonal relatedness of colistin-resistant strains producing KPC-type carbapenemase (KPC-KP) causing subsequent infections or colonization. Moreover, we aimed to gain insight into the ability of biofilm production in strains producing carbapenemase. Twenty-two consecutive KPC-KP and one KPC-negative strain was identified from an adult intensive care unit in Brazil. Seventy-five percent of isolates that harbored the gene exhibited genetic relatedness by pulsed-field gel electrophoresis, and none presented the plasmid-mediated and genes. This study showed that the majority of repeated KPC infections in adults were caused by a clone that caused the previous infections/colonizations even after a long period of time and illustrates the capacity of multiple clones producing biofilms to coexist in the same patient at the same time, becoming a reservoir of KPC-KP in the hospital environment.
产碳青霉烯酶的生物体具有传染性，对公共健康构成重大威胁。我们研究了导致后续感染或定植的产 KPC 型碳青霉烯酶（KPC-KP）的多粘菌素耐药株的克隆相关性。此外，我们旨在深入了解产碳青霉烯酶菌株的生物膜产生能力。从巴西的一个成人重症监护病房连续鉴定出 22 株产 KPC-KP 和 1 株 KPC 阴性菌株。通过脉冲场凝胶电泳，携带 基因的 75%的分离株显示出遗传相关性，且均未显示质粒介导的 和 基因。本研究表明，成人中多次重复的 KPC 感染大多是由同一克隆引起的，即使在很长一段时间后，该克隆也会引起先前的感染/定植，这说明了能够产生生物膜的多个克隆同时在同一患者体内共存的能力，成为医院环境中 KPC-KP 的储库。
