Normandie University, UNICAEN, Sorbonne Universités, MNHN, UPMC Univ Paris 06, UA, CNRS, IRD, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie, Caen, 14032, France.
Aquativ (DianaAqua, Symrise Group), Elven, France.
BMC Genomics. 2018 May 24;19(1):396. doi: 10.1186/s12864-018-4780-0.
Aquaculture production is expected to double by 2030, and demands for aquafeeds and raw materials are expected to increase accordingly. Sustainable growth of aquaculture will require the development of highly nutritive and functional raw materials to efficiently replace fish meal. Enzymatic hydrolysis of marine and aquaculture raw materials could bring new functionalities to finished products. The aim of this study was to determine the zootechnical and transcriptomic performances of protein hydrolysates of different origins (tilapia, shrimp, and a combination of the two) in European seabass (Dicentrarchux labrax) fed a low fish meal diet (5%), for 65 days.
Results were compared to a positive control fed with 20% of fish meal. Growth performances, anterior intestine histological organization and transcriptomic responses were monitored and analyzed. Dietary inclusion of protein hydrolysates in the low fish meal diet restored similar growth performances to those of the positive control. Inclusion of dietary shrimp hydrolysate resulted in larger villi and more goblet cells, even better than the positive control. Transcriptomic analysis of the anterior intestine showed that dietary hydrolysate inclusion restored a pattern of intestinal gene expression very close to the pattern of the positive control. However, as compared to the low fish meal diet and depending on their origin, the different hydrolysates did not modulate metabolic pathways in the same way. Dietary shrimp hydrolysate inclusion modulated more metabolic pathways related to immunity, while nutritional metabolism was more impacted by dietary tilapia hydrolysate. Interestingly, the combination of the two hydrolysates enhanced the benefits of hydrolysate inclusion in diets: more genes and metabolic pathways were regulated by the combined hydrolysates than by each hydrolysate tested independently.
Protein hydrolysates manufactured from aquaculture by-products are promising candidates to help replace fish meal in aquaculture feeds without disrupting animal metabolism and performances.
预计到 2030 年，水产养殖产量将翻一番，对水产饲料和原材料的需求也将相应增加。水产养殖业的可持续增长需要开发高营养和功能性的原材料，以有效地替代鱼粉。对海洋和水产养殖原材料进行酶解可以为成品带来新的功能。本研究的目的是确定不同来源（罗非鱼、虾和两者的组合）的蛋白质水解产物在投喂低鱼粉饲料（5%）的欧洲海鲈（Dicentrarchux labrax）中的生产性能和转录组表现，为期 65 天。
将结果与投喂 20%鱼粉的阳性对照组进行比较。监测和分析了生长性能、前肠组织学结构和转录组反应。在低鱼粉饲料中添加蛋白质水解产物可以恢复与阳性对照组相似的生长性能。添加虾水解产物的饮食导致绒毛更大，杯状细胞更多，甚至优于阳性对照组。前肠的转录组分析表明，饮食中添加水解产物可以恢复与阳性对照组非常相似的肠道基因表达模式。然而，与低鱼粉饲料相比，根据其来源的不同，不同的水解产物不会以相同的方式调节代谢途径。虾水解产物的添加更多地调节了与免疫相关的代谢途径，而营养代谢则更多地受到饲料罗非鱼水解产物的影响。有趣的是，两种水解产物的组合增强了水解产物在饲料中的添加效果：与单独测试的每种水解产物相比，组合水解产物调节了更多的基因和代谢途径。
由水产养殖副产物制成的蛋白质水解产物是有希望的候选物，可以帮助替代水产养殖饲料中的鱼粉，而不会干扰动物的新陈代谢和表现。
Zotero 插件
