Rodríguez-Cal Y Mayor Arianna, Cruz-Antonio Leticia, Castañeda-Hernández Gilberto, Favari-Perozzi Liliana, Guízar-Sahagún Gabriel
Departament of Pharmacology, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico.
Departament of Pharmacy, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Spinal Cord. 2018 Oct;56(10):964-970. doi: 10.1038/s41393-018-0144-5. Epub 2018 May 23.
This was an animal study.
Local inflammation is attenuated below high thoracic SCI, where innervation of major lymphoid organs is involved. However, whether inflammatory responses are affected after low thoracic SCI, remains undetermined. The aim of this study was to characterize the influence of low thoracic SCI on carrageenan-induced paw swelling in intact and paralyzed limbs, at acute and subacute stages.
University and hospital-based research center, Mexico City, Mexico.
Rats received a severe contusive SCI at T9 spinal level or sham injury. Then, 1 and 15 days after lesion, carrageenan or vehicle was subcutaneously injected in forelimb and hindlimb paws. Paw swelling was measured over a 6-h period using a plethysmometer.
Swelling increased progressively reaching the maximum 6 h post-carrageenan injection. Swelling increase in sham-injured rats was approximately 130% and 70% compared with baseline values of forelimbs and hindlimbs, respectively. Paws injected with saline exhibited no measurable swelling. Carrageenan-induced paw swelling 1-day post-SCI was suppressed in both intact and paralyzed limbs. Fifteen days post-injury, the swelling response to carrageenan was completely reestablished in forelimbs, whereas in hindlimbs it remained significantly attenuated compared with sham-injured rats.
SCI at low spinal level affects the induced swelling response in a different way depending on both, the neurological status of challenged regions and the stage of injury. These findings suggest that neurological compromise of the main immunological organs is not a prerequisite for the local swelling response to be affected after injury.
这是一项动物研究。
在涉及主要淋巴器官神经支配的高胸段脊髓损伤下方，局部炎症会减轻。然而，胸段低位脊髓损伤后炎症反应是否受到影响仍未确定。本研究的目的是在急性和亚急性阶段，描述胸段低位脊髓损伤对完整和瘫痪肢体中角叉菜胶诱导的爪肿胀的影响。
墨西哥城基于大学和医院的研究中心。
大鼠在T9脊髓水平接受严重的挫伤性脊髓损伤或假损伤。然后，在损伤后1天和15天，将角叉菜胶或赋形剂皮下注射到前肢和后肢爪中。使用体积描记器在6小时内测量爪肿胀情况。
肿胀逐渐增加，在注射角叉菜胶后6小时达到最大值。与前肢和后肢的基线值相比，假损伤大鼠的肿胀增加分别约为130%和70%。注射生理盐水的爪没有可测量的肿胀。脊髓损伤后1天，角叉菜胶诱导的爪肿胀在完整和瘫痪肢体中均受到抑制。损伤后15天，前肢对角叉菜胶的肿胀反应完全恢复，而后肢与假损伤大鼠相比仍明显减弱。
低位脊髓损伤以不同方式影响诱导的肿胀反应，这取决于受挑战区域的神经状态和损伤阶段。这些发现表明，主要免疫器官的神经损伤不是损伤后局部肿胀反应受影响的先决条件。
