Nicolas L, Darriet F, Hougard J M
OCCGE/ORSTROM Institut Pierre Richet, Bouaké, Ivory Coast.
Med Vet Entomol. 1987 Apr;1(2):157-62. doi: 10.1111/j.1365-2915.1987.tb00337.x.
A flowable concentrate of Bacillus sphaericus (Neide) strain 2362 was applied against Anopheles gambiae Giles s.l. mosquito larve in small plot field trials in Bobo-Dioulasso area. Burkina-Faso. Third and fourth instar larvae were controlled for 10-15 days with a dosage of 10 g/m2, 3-10 days with 1 or 0.1 mg/m2, and 2 days with 0.01 g/m2. Complete elimination of larval populations required 1 x 10(2) to 2 x 10(3) viable spores/ml in the larval feeding zone. After treatment, the total numbers of viable spores decreased in the ponds, due to ingestion of spores by non-target as well as target organisms and/or loss of viability of some spores by sunlight. This formulation was less effective against An. gambiae than against Culex quinquefasciatus Say larvae, both in laboratory bioassays and under field conditions.
球形芽孢杆菌（奈德氏）菌株2362的一种可流动浓缩物，在布基纳法索博博迪乌拉索地区的小块田间试验中，被用于防治冈比亚按蚊复合组的蚊虫幼虫。用10克/平方米的剂量可使三龄和四龄幼虫得到10至15天的控制，用1毫克/平方米或0.1毫克/平方米的剂量可控制3至10天，用0.01克/平方米的剂量可控制2天。在幼虫取食区，要完全消灭幼虫种群需要每毫升1×10²至2×10³个活孢子。处理后，池塘中活孢子的总数减少，这是由于非靶标以及靶标生物摄取孢子和/或一些孢子因阳光照射而丧失活力所致。在实验室生物测定和田间条件下，这种制剂对冈比亚按蚊的防治效果比对致倦库蚊幼虫的防治效果差。
