Pierzynowska Karolina, Rzeszótko Agata, Blendowska Aleksandra, Wieczerzak Ewa, Rodziewicz-Motowidło Sylwia, Piotrowska Ewa, Węgrzyn Grzegorz
Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Gdańsk, Poland.
Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland.
Acta Biochim Pol. 2018;65(2):325-332. doi: 10.18388/abp.2018_2596. Epub 2018 May 22.
Flavonoids, polyphenolic compounds present in many food products, affect growth of different bacterial species when tested as purified or synthetic substances. They can also influence gene expression in human cells, like fibroblasts. Here, we asked if soy isoflavone extracts, commonly used in many products sold as anti-menopausal dietary supplements, influence bacterial growth similarly to a synthetic isoflavone, genistein. Four commercially available products were tested in amounts corresponding to genistein concentrations causing inhibition of growth of Vibrio harveyi (a model bacterium sensitive to this isoflavone) and Escherichia coli (a model bacterium resistant to genistein). Differential effects of various extracts on V. harveyi and E. coli growth, from stimulation, to no changes, to inhibition, were observed. Moreover, contrary to genistein, the tested extracts caused a decrease (to different extent) in viability of human dermal fibroblasts. These results indicate that effects of various soy isoflavone extracts on bacterial growth and viability of human cells are different, despite similar declared composition of the commercially available products.
黄酮类化合物是存在于许多食品中的多酚类化合物，当作为纯化或合成物质进行测试时，它们会影响不同细菌种类的生长。它们还可以影响人类细胞（如成纤维细胞）中的基因表达。在此，我们探讨了许多作为抗更年期膳食补充剂销售的产品中常用的大豆异黄酮提取物，是否与合成异黄酮染料木黄酮一样影响细菌生长。对四种市售产品进行了测试，其用量对应于能抑制哈维氏弧菌（一种对这种异黄酮敏感的模式细菌）和大肠杆菌（一种对染料木黄酮具有抗性的模式细菌）生长的染料木黄酮浓度。观察到各种提取物对哈维氏弧菌和大肠杆菌生长的不同影响，从刺激到无变化再到抑制。此外，与染料木黄酮相反，测试的提取物导致人皮肤成纤维细胞的活力下降（程度不同）。这些结果表明，尽管市售产品宣称的成分相似，但各种大豆异黄酮提取物对细菌生长和人类细胞活力的影响是不同的。
