Institute of Molecular and Translation Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, Olomouc 779 00, Czech Republic.
Department of Organic Chemistry, Faculty of Science, Palacky University Olomouc, 17. Listopadu 12, Olomouc 771 46, Czech Republic.
Chem Commun (Camb). 2018 Jul 14;54(55):7589-7592. doi: 10.1039/c8cc01731j. Epub 2018 May 24.
A synthetic three-fluorophore system with two enzymatically cleavable linkers has been developed for the simultaneous detection of two proteases in a mixture. The probe was designed to afford single excitation/triple emission ratiometric detection through a fluorescence change during the cleavage of a peptide linker. The developed assays were verified for trypsin and chymotrypsin as the model enzymes.
已经开发出一种带有两个酶切连接子的合成三荧光团系统，用于混合物中两种蛋白酶的同时检测。该探针设计为通过肽连接子切割过程中的荧光变化提供单激发/三重发射比率检测。已经验证了该测定法用于胰蛋白酶和糜蛋白酶作为模型酶。
