The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.
Chem Soc Rev. 2020 Aug 7;49(15):5110-5139. doi: 10.1039/c9cs00318e. Epub 2020 Jul 22.
In this tutorial review, we will explore recent advances in the construction and application of Förster resonance energy transfer (FRET)-based small-molecule fluorescent probes. The advantages of FRET-based fluorescent probes include: a large Stokes shift, ratiometric sensing and dual/multi-analyte responsive systems. We discuss the underlying energy donor-acceptor dye combinations and emphasise their applications for the detection or imaging of cations, anions, small neutral molecules, biomacromolecules, cellular microenvionments and dual/multi-analyte responsive systems.
在本教程综述中,我们将探讨基于Förster 共振能量转移 (FRET) 的小分子荧光探针的构建和应用的最新进展。基于 FRET 的荧光探针的优点包括:大斯托克斯位移、比率感应和双/多分析物响应系统。我们讨论了潜在的供体-受体染料组合,并强调了它们在阳离子、阴离子、小分子中性分子、生物大分子、细胞微环境和双/多分析物响应系统的检测或成像中的应用。