College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
Chem Commun (Camb). 2018 Jun 21;54(51):6991-6994. doi: 10.1039/c8cc03769h.
We develop a label-free and high-throughput bioluminescence method for the sensitive detection of uracil DNA glycosylase (UDG) through enzyme-mediated tricyclic cascade signal amplification. This method exhibits high sensitivity with a detection limit as low as 0.00031 U mL-1, and it can be further applied for the measurement of enzyme kinetic parameters and the screening of UDG inhibitors as well as cancer cell analysis.
我们开发了一种无标记、高通量的生物发光方法，用于通过酶介导的三环级联信号放大灵敏检测尿嘧啶 DNA 糖基化酶 (UDG)。该方法具有很高的灵敏度，检测限低至 0.00031 U mL-1，可进一步用于酶动力学参数的测量、UDG 抑制剂的筛选以及癌细胞分析。
