College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
Chem Commun (Camb). 2020 Mar 10;56(20):2971-2974. doi: 10.1039/c9cc09999a.
We demonstrate for the first time that host-guest recognition coupled with triple signal amplification endows an electrochemiluminescent (ECL) biosensor with enhanced sensitivity for uracil DNA glycosylase (UDG) assay. This biosensor exhibits good selectivity and extremely high sensitivity, and it can be used to screen UDG inhibitors and measure the cellular UDG activity as well.
我们首次证明，主体-客体识别与三重信号放大相结合，赋予电化学发光（ECL）生物传感器用于尿嘧啶 DNA 糖基化酶（UDG）检测的增强灵敏度。该生物传感器表现出良好的选择性和极高的灵敏度，可用于筛选 UDG 抑制剂和测量细胞 UDG 活性。
