小鼠主嗅觉上皮中嗅觉受体的近乎完整的空间图谱。
A Near-Complete Spatial Map of Olfactory Receptors in the Mouse Main Olfactory Epithelium.
机构信息
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Systems Biology PhD Program, Harvard Medical School, Boston, MA, USA.
出版信息
Chem Senses. 2018 Jul 5;43(6):427-432. doi: 10.1093/chemse/bjy030.
Abstract
Different regions of the mammalian nose smell different odors. In the mouse olfactory system, spatially regulated expression of >1000 olfactory receptors (ORs) along the dorsomedial-ventrolateral (DV) axis forms a topological map in the main olfactory epithelium (MOE). However, the locations of most ORs along the DV axis are currently unknown. By sequencing mRNA of 12 isolated MOE pieces, we mapped out the DV locations-as quantified by "zone indices" on a scale of 1-5-of 1033 OR genes with an estimated error of 0.3 zone indices. Our map covered 81% of all intact OR genes and 99.4% of the total OR mRNA abundance. Spatial regulation tended to vary gradually along chromosomes. We further identified putative non-OR genes that may exhibit spatial expression along the DV axis.
摘要
哺乳动物的不同鼻腔区域能嗅出不同的气味。在老鼠嗅觉系统中，上千个嗅觉受体（OR）沿背侧-腹侧（DV）轴的空间调控表达，在主嗅觉上皮（MOE）中形成一个拓扑图谱。然而，目前大多数 OR 沿 DV 轴的位置尚不清楚。通过对 12 个分离的 MOE 片段的 mRNA 进行测序，我们绘制出了 1033 个 OR 基因的 DV 位置（用 1-5 的“区指数”量化），其估计误差为 0.3 个区指数。我们的图谱覆盖了所有完整的 OR 基因的 81%和总 OR mRNA 丰度的 99.4%。空间调控往往沿着染色体逐渐变化。我们还进一步鉴定了可能沿 DV 轴表达的假定非 OR 基因。
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