Pecková Radka, Sak Bohumil, Květoňová Dana, Kváč Martin, Koriťáková Eva, Foitová Ivona
Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic.
Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, v.v.i., Branišovská 31, 37005, České Budějovice, Czech Republic.
Parasitol Res. 2018 Aug;117(8):2437-2443. doi: 10.1007/s00436-018-5932-5. Epub 2018 May 24.
Fifteen Mongolian gerbils were inoculated with 10 × 10 viable trophozoites of Giardia intestinalis. Their faeces were examined daily by flotation method and the number of shed cysts was counted. Two animals (male and female) were euthanised at 4- to 5-day intervals (9, 14, 18 days post-infection (DPI)). The remaining nine gerbils were sacrificed and dissected at the end of the experiment (23 DPI). Their small intestinal tissues were processed for examination using histological sectioning and scanning electron microscopy and their complete blood count (CBC) was examined. The highest number of trophozoites at the total was observed in the duodenum in gerbils sacrificed on 14 DPI. Number of shed cysts was positively correlated with number of trophozoites rinsed from the intestine. Infected gerbils had lower body weight gain in comparison with control group and in three male gerbils; diarrhoea occurred during infection. Cyst shedding was negatively correlated with values of mean corpuscular haemoglobin concentration. Females showed another pattern in cyst shedding than males. This information needs to be taken into account while planning the experiments.
15只蒙古沙鼠接种了10×10个活的肠贾第鞭毛虫滋养体。每天通过漂浮法检查它们的粪便，并计算排出的包囊数量。每隔4至5天（感染后9、14、18天）对两只动物（一雄一雌）实施安乐死。在实验结束时（感染后23天）处死并解剖其余9只沙鼠。对它们的小肠组织进行组织切片和扫描电子显微镜检查，并检查其全血细胞计数（CBC）。在感染后14天处死的沙鼠中，十二指肠中观察到的滋养体总数最高。排出的包囊数量与从肠道冲洗出的滋养体数量呈正相关。与对照组相比，感染的沙鼠体重增加较低，并且在三只雄性沙鼠中，感染期间出现腹泻。包囊排出与平均红细胞血红蛋白浓度值呈负相关。雌性在包囊排出方面表现出与雄性不同的模式。在规划实验时需要考虑这些信息。
