Reynoso-Robles R, Ponce-Macotela M, Rosas-López L E, Ramos-Morales A, Martínez-Gordillo M N, González-Maciel A
Laboratorio de Morfologia Celular y Tisular (Laboratory of Cell and Tissue Morphology), Instituto Nacional de Pediatría (Mexican National Institute of Paediatrics), Insurgentes Sur No. 3700-C, Mexico, D. F. C. P. 04530, Mexico.
Laboratorio de Parasitología Experimental (Laboratory of Experimental Parasitology), Instituto Nacional de Pediatría (Mexican National Institute of Paediatrics).
Sci Rep. 2015 Oct 16;5:15168. doi: 10.1038/srep15168.
Giardiasis is a neglected parasitic disease that affects primarily children, in whom it delays physical and mental development. The pathophysiology of giardiasis in not well understood, and most reports have identified Giardia intestinalis trophozoites only in the lumen and on the brush border of the small intestine. We identified Giardia trophozoites within the epithelium of the small intestine of a lactose intolerance patient. The Giardia trophozoites were obtained and cultured in vitro. In addition, we demonstrated Giardia trophozoite invasion in an animal model. Giardia trophozoites invaded the intestinal mucosa and submucosa of infected gerbils. The invasive trophozoites were observed at 21, 30 and 60 days age, and the average numbers of invaded sites were 17 ± 5, 15 ± 4, and 9 ± 3, respectively. We found trophozoites between epithelial cells, at the base of empty goblet cells, in lacteal vessels and within the submucosa. The morphological integrity of the invasive trophozoites was demonstrated via electron microscopy. The analysis of the gerbils infected with the trophozoites of the WB reference strain did not show intraepithelial trophozoites. These results demonstrate another Giardia pathogenic mechanism, opening the door to numerous future studies.
贾第虫病是一种被忽视的寄生虫病，主要影响儿童，会延缓其身心发育。贾第虫病的病理生理学尚未完全了解，大多数报告仅在小肠腔和小肠刷状缘发现了肠贾第虫滋养体。我们在一名乳糖不耐受患者的小肠上皮内发现了贾第虫滋养体。获取了贾第虫滋养体并进行体外培养。此外，我们在动物模型中证明了贾第虫滋养体的侵袭。贾第虫滋养体侵袭了受感染沙鼠的肠黏膜和黏膜下层。在21天、30天和60天龄时观察到侵袭性滋养体，侵袭部位的平均数量分别为17±5、15±4和9±3。我们在上皮细胞之间、空杯状细胞底部、乳糜管内和黏膜下层发现了滋养体。通过电子显微镜证实了侵袭性滋养体的形态完整性。对感染WB参考株滋养体的沙鼠的分析未显示上皮内滋养体。这些结果证明了贾第虫的另一种致病机制，为未来的众多研究打开了大门。
