Alapat Daisy
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Methods Mol Biol. 2018;1792:35-45. doi: 10.1007/978-1-4939-7865-6_3.
Cytoplasmic immunoglobulin (CIg) vs DNA by flow cytometric (FCM) method allows us to detect DNA content of the neoplastic plasma cells with monotypic cytoplasmic immunoglobulin and also provide us with clear distinction from normal polytypic plasma cells. Abnormalities in cellular DNA content (DNA aneuploidy) and cell cycle determination (proliferative activity) can be measured rapidly by flow cytometry. FCM can measure gross differences in DNA content and distinguish a cell population with normal DNA content, or diploid from a cell population with an abnormal or aneuploid DNA content. With the dual parameter procedure a monoclonal plasma cell population can be identified down to less than 0.1-0.05% of all cells.
通过流式细胞术（FCM）检测细胞质免疫球蛋白（CIg）与DNA，使我们能够检测具有单型细胞质免疫球蛋白的肿瘤性浆细胞的DNA含量，还能将其与正常多型性浆细胞清晰区分开来。通过流式细胞术可快速测量细胞DNA含量异常（DNA非整倍体）和细胞周期测定（增殖活性）。FCM可以测量DNA含量的总体差异，并区分DNA含量正常或二倍体的细胞群与DNA含量异常或非整倍体的细胞群。采用双参数程序，可以识别出占所有细胞不到0.1 - 0.05%的单克隆浆细胞群。
