Chan C S, Wormsley S B, Peter J B, Schechter G P
Hematology Section, Veterans Administration Medical Center, Washington, D.C. 20422.
Am J Clin Pathol. 1989 Jan;91(1):12-7. doi: 10.1093/ajcp/91.1.12.
Dual-parameter flow cytometric analysis of monotypic cytoplasmic immunoglobulin (CIg) and DNA content on 15 myeloma marrows allowed S-phase determination of the CIg(+) tumor separately from the CIg(-) hematopoietic cell pool. The median percentage of the CIg(+) cells in S-phase was 2% compared with 5% for the CIg(-) cells. The median survival of patients with more than 2%, and those with 2% or less CIg(+) S-phase cells was 2 months and more than 13 months, respectively, from the time of study. Ploidy analysis identified four patterns of plasma cell DNA content: entirely hyperdiploid, entirely diploid, combined diploid and tetraploid stemlines, and tumors containing diploid and aneuploid CIg(+) cells. A monotypic CIg(+) double stemline myeloma was distinguished from two aneuploid tumors containing admixed normal, diploid polyclonal plasma cells. This technique provides an improved and expedient means for determining the proliferating fractions of myeloma cells and enhances recognition of double stemline tumors and clonal evolution in myeloma.
对15例骨髓瘤骨髓进行单型细胞质免疫球蛋白（CIg）和DNA含量的双参数流式细胞术分析，能够分别测定CIg（+）肿瘤细胞的S期，与CIg（-）造血细胞池区分开来。S期CIg（+）细胞的中位数百分比为2%，而CIg（-）细胞为5%。从研究开始时起，CIg（+）S期细胞超过2%的患者和CIg（+）S期细胞为2%或更低的患者的中位生存期分别为2个月和超过13个月。倍性分析确定了浆细胞DNA含量的四种模式：完全超二倍体、完全二倍体、二倍体和四倍体干细胞系组合，以及含有二倍体和非整倍体CIg（+）细胞的肿瘤。一种单型CIg（+）双干细胞系骨髓瘤与两种含有混合正常二倍体多克隆浆细胞的非整倍体肿瘤区分开来。该技术为确定骨髓瘤细胞的增殖分数提供了一种改进且便捷的方法，并增强了对双干细胞系肿瘤和骨髓瘤克隆进化的识别。
