Hoffman Jessica R, Tasciotti Ennio, Molinaro Roberto
University of North Carolina, Chapel Hill, NC, USA.
Center of Biomimetic Medicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA.
Methods Mol Biol. 2018;1792:205-214. doi: 10.1007/978-1-4939-7865-6_15.
Liposomes used for the delivery of pharmaceuticals have difficulties scaling up and reaching clinical translation as they suffer from batch-to-batch variability. Here, we describe a microfluidic approach for creating reproducible, homogenous nanoparticles with tunable characteristics. These nanoparticles of sizes ranging from 30 to 500 nm are rapidly self-assembled by controlling the flow rates of ethanol and aqueous streams. This method of microfluidic assembly allows for the efficient encapsulation of both hydrophobic and hydrophilic drugs in the lipid bilayer and particle core, respectively, either separately or in combination.
用于药物递送的脂质体在扩大规模和实现临床转化方面存在困难，因为它们存在批次间的差异。在此，我们描述了一种微流控方法，用于创建具有可调特性的可重复、均匀的纳米颗粒。通过控制乙醇和水流的流速，可快速自组装出尺寸范围为30至500纳米的这些纳米颗粒。这种微流控组装方法能够分别或联合将疏水性和亲水性药物高效地封装在脂质双层和颗粒核心中。
