Department of Experimental Medicine, Sapienza University, Rome, Italy.
Department of Anatomical, Histological, Forensic, and Orthopaedic Sciences, Sapienza University, Rome, Italy.
J Cell Physiol. 2018 Nov;233(11):8411-8417. doi: 10.1002/jcp.26796. Epub 2018 May 24.
Different adipose tissue (AT) depots are associated with multiple metabolic risks. Phosphodiesterase type 5 (PDE5) is involved in adipocyte physiology and PDE5 inhibition may affect adipogenesis and ameliorate white AT quality. The aim of this study is to investigate the distribution of AT and the composition of the stroma-vascular fraction (SVF) of subcutaneous AT (SAT) in type 2 diabetic mice after prolonged treatment with a PDE5 inhibitor, Sildenafil. 18 db/db mice were treated with Sildenafil or vehicle for 12 weeks. AT distribution was monitored and SAT was processed for isolation of SVF by flow cytometry. Sildenafil induced an overall reduction in AT, mainly in visceral AT (VAT), compared with SAT. In Sildenafil-treated mice, the mean change in body weight from baseline positively correlated with VAT, but not with SAT. Characterization of SVF of SAT showed an increase in the frequency of M2 macrophages and endothelial cells in treated mice. Sildenafil improved the maintenance of SAT homeostasis and distribution.
不同的脂肪组织（AT）与多种代谢风险相关。磷酸二酯酶 5（PDE5）参与脂肪细胞的生理过程，PDE5 抑制可能影响脂肪生成并改善白色 AT 的质量。本研究旨在探讨在长期使用 PDE5 抑制剂西地那非治疗 2 型糖尿病小鼠后，其皮下脂肪组织（SAT）的 AT 分布和基质血管部分（SVF）的组成。18 只 db/db 小鼠接受西地那非或载体治疗 12 周。监测 AT 分布，并通过流式细胞术分离 SAT 的 SVF。与 SAT 相比，西地那非治疗导致整体脂肪量减少，主要是内脏脂肪（VAT）减少。在西地那非治疗的小鼠中，从基线开始的体重平均变化与 VAT 呈正相关，但与 SAT 无关。SAT 的 SVF 特征表明，治疗小鼠中 M2 巨噬细胞和内皮细胞的频率增加。西地那非改善了 SAT 体内平衡和分布的维持。
