Fávero Juscivete Fátima, Da Silva Aleksandro S, Bottari Nathieli B, Schetinger Maria Rosa C, Morsch Vera Maria M, Baldissera Matheus D, Stefani Lenita M, Machado Gustavo
Graduate Program of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
J Anim Physiol Anim Nutr (Berl). 2018 Aug;102(4):910-916. doi: 10.1111/jpn.12917. Epub 2018 May 24.
The aim of this study was to evaluate the behaviour of adenosine deaminase (ADA) activity, as well as its participation in the immunomodulation of pregnant cows. Thus, sixteen cows were divided into two groups (A and B): the group A was composed by cows not pregnant (n = 8), while the group B was composed by pregnant cows (n = 8). Serum levels of interleukin-10 (IL-10), IL-6, tumour necrosis factor alpha (TNF-α), reactive oxygen species (ROS) and C-reactive protein (CRP), as well as ADA and glutathione S-transferase (GST) activities, were measured on five sampling times (3, 5, 7 and 8 months of gestation, and soon after calving). Serum ADA activity was similar throughout the experiment in the cows belonging to the group A, but its activity increased during the experiment in cows from the group B, that is it was lower in the third and fifth months of pregnancy, and higher on months 7, 8 and after calving when compared to the group A. TNF-α and IL-6 serum levels were lower in pregnant cows compared to non-pregnant animals; however, they significantly increased after calving. Serum levels of IL-10 increased after 8 months of gestation, but it reduced after calving when compared to the group A, while CRP increased on month 8 of gestation and after calving compared to the group A. Pregnant cows showed lower serum ROS levels on months 3, 5 and 7 of gestation, and higher levels at the post-partum. Serum GST activity was higher on month 5 of gestation in pregnant cows, but it was lower on months 7, 8 and in the post-partum compared to the group A. Based on these evidence, we concluded that ADA activity and the others mediators or inflammatory modulators have important role in the maintenance of cow's gestation due to their immunomodulatory effects.
本研究的目的是评估腺苷脱氨酶（ADA）活性的变化情况，以及其在妊娠母牛免疫调节中的作用。因此，将16头母牛分为两组（A组和B组）：A组由未怀孕的母牛组成（n = 8），而B组由怀孕的母牛组成（n = 8）。在五个采样时间点（妊娠3、5、7和8个月以及产犊后不久）测量血清白细胞介素-10（IL-10）、IL-6、肿瘤坏死因子α（TNF-α）、活性氧（ROS）和C反应蛋白（CRP）的水平，以及ADA和谷胱甘肽S-转移酶（GST）的活性。在整个实验过程中，A组母牛的血清ADA活性相似，但B组母牛的ADA活性在实验过程中有所增加，即在妊娠的第三个月和第五个月较低，而在第7、8个月以及与A组相比产犊后的ADA活性较高。与未怀孕的动物相比，怀孕母牛的TNF-α和IL-6血清水平较低；然而，产犊后它们显著升高。妊娠8个月后，IL-10的血清水平升高，但与A组相比，产犊后降低，而与A组相比，妊娠第8个月和产犊后的CRP升高。怀孕母牛在妊娠第3、5和第7个月的血清ROS水平较低，产后水平较高。怀孕母牛在妊娠第5个月时血清GST活性较高，但与A组相比，在第7、8个月和产后较低。基于这些证据，我们得出结论，ADA活性以及其他介质或炎症调节剂因其免疫调节作用在维持母牛妊娠中具有重要作用。
