Department of Biochemistry, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Vet Med Sci. 2024 Nov;10(6):e70023. doi: 10.1002/vms3.70023.
Thiol/disulphide homeostasis (TDH) has a critical role in many cellular activities such as antioxidant protection. Alterations of oxidative stress in the transition period play an important role in development of some diseases and disorders in dairy cows.
The purpose of this study is to assess the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), total thiol, native thiol, disulphide and lipid profile in Simmental and Montofon dairy cows (aged 2-3) before and after calving.
Blood samples were collected 233-280 days of pregnancy and the 30 days of post-partum. Serum total thiol, native thiol and disulphide levels were determined as well as TAS, TOS and paraoxonase-1 (PON-1) levels were measured using colorimetric assays. Triglycerides (TG), total cholesterol, HDL cholesterol and LDL cholesterol levels were measured with an automatic analyser.
Total thiol (p = 0.038) and disulphide (p = 0.015) levels were higher after calving compare to pregnancy in Montofon. TAS was found lower (p < 0.001), and OSI was higher in both breeds (Montofon p = 0.012, Simmental p = 0.028) after calving than in pregnancy. When compared between pregnancy and after calving levels in the same breed, HDL was found to be higher after calving (p < 0.001) and TG was lower after calving (p = 0.020) in Montofon. PON (p = 0.090), HDL (p < 0.001) and cholesterol levels were found higher (p < 0.001) and TG level was lower (p < 0.001) after calving in Simmental.
According to our results, we observed different responses between two breeds before and after calving. There are few studies about TDH in animal research, and this is the first study in the literature that evaluates the TDH along with oxidative stress and lipid profiles in dairy cows in the periparturient and post-partum period.
硫醇/二硫键平衡（TDH）在许多细胞活动中起着关键作用，如抗氧化保护。过渡期氧化应激的改变在奶牛的一些疾病和疾病的发展中起着重要作用。
本研究旨在评估西门塔尔和蒙托丰奶牛（2-3 岁）在分娩前后的总抗氧化状态（TAS）、总氧化状态（TOS）、氧化应激指数（OSI）、总巯基、天然巯基、二硫键和脂质谱。
在妊娠 233-280 天和产后 30 天采集血样。用比色法测定血清总巯基、天然巯基和二硫键水平，以及 TAS、TOS 和对氧磷酶-1（PON-1）水平。用自动分析仪测定甘油三酯（TG）、总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇水平。
蒙托丰牛产后总巯基（p=0.038）和二硫键（p=0.015）水平高于妊娠。产后两种牛的 TAS 均较低（p<0.001），OSI 均较高（蒙托丰 p=0.012，西门塔尔 p=0.028）。与同一品种妊娠和产后相比，蒙托丰产后 HDL 较高（p<0.001），产后 TG 较低（p=0.020）。蒙托丰的 PON（p=0.090）、HDL（p<0.001）和胆固醇水平较高（p<0.001），产后 TG 水平较低（p<0.001）。
根据我们的结果，我们观察到两个品种在分娩前后有不同的反应。动物研究中关于 TDH 的研究很少，这是文献中首次评估围产期和产后奶牛的 TDH 以及氧化应激和脂质谱。
