Spasic Jelena, Mandic Mina, Radivojevic Jelena, Jeremic Sanja, Vasiljevic Branka, Nikodinovic-Runic Jasmina, Djokic Lidija
Institute of Molecular Genetics and Genetic Engineering , University of Belgrade, Belgrade, Serbia.
Biotechnol Appl Biochem. 2018 Nov;65(6):822-833. doi: 10.1002/bab.1664. Epub 2018 Jun 6.
Biocatalytic potential of Streptomyces strains isolated from the rhizosphere of plants and from mycorrhizosphere of fungi has been investigated. A total of 118 Streptomyces isolates were selected and functionally screened for 10 different biotechnologically important enzymatic activities: hydrolase (cellulase, cutinase, gelatinase, lipase, protease, polyhydroxyalkanoate (PHA) depolymerase), phenol oxidase and peroxidase (laccase, tyrosinase, and lignin peroxidase), and aminotransferase. Out of 118 tested Streptomyces spp., 90% showed at least one enzymatic activity. The most abundant were enzymes involved in the biomass degradation, as the production of cutinase, cellulase, and lignin peroxidase were detected in 31%, 40%, and 48% of the isolates, respectively. The improved specific activities of lipase (isolates BV315 and BV100) and tyrosinase (isolates BV87 and BV88) were shown in comparison with the industrially relevant activities of Pseudomonas strains. Plant rhizosphere soils were more prolific source of Streptomyces strains with biocatalytic potential in comparison with mycorrhizosphere soils. Overall, 284 enzyme activities among 118 Streptomyces isolates have been detected. This is the first comprehensive screening of Streptomyces isolates from rhizosphere and mycorrhizosphere soils for novel biocatalysts, showing that specific environmental habitats, such as rhizosphere soils, are "treasure troves" of Streptomyces with biocatalytic potential.
对从植物根际和真菌菌根际分离出的链霉菌菌株的生物催化潜力进行了研究。共选择了118株链霉菌分离株，并对10种不同的具有重要生物技术意义的酶活性进行了功能筛选：水解酶（纤维素酶、角质酶、明胶酶、脂肪酶、蛋白酶、聚羟基脂肪酸酯（PHA）解聚酶）、酚氧化酶和过氧化物酶（漆酶、酪氨酸酶和木质素过氧化物酶）以及转氨酶。在118株受试链霉菌中，90%至少表现出一种酶活性。最丰富的是参与生物质降解的酶，分别在31%、40%和48%的分离株中检测到角质酶、纤维素酶和木质素过氧化物酶的产生。与假单胞菌菌株的工业相关活性相比，脂肪酶（分离株BV315和BV100）和酪氨酸酶（分离株BV87和BV88）的比活性有所提高。与菌根际土壤相比，植物根际土壤是具有生物催化潜力的链霉菌菌株的更丰富来源。总体而言，在118株链霉菌分离株中检测到284种酶活性。这是首次对来自根际和菌根际土壤的链霉菌分离株进行全面筛选以寻找新型生物催化剂，表明特定的环境栖息地，如根际土壤，是具有生物催化潜力的链霉菌的“宝库”。
