Jouanneau J, Bertrand S, Ducros E, Longuet M, Dore J F, Tavitian A
U248 INSERM Faculté de Médecine Lariboisière-Saint-Louis, Paris, France.
In Vivo. 1987 Mar-Apr;1(2):119-24.
The oncogenic potentialities of human melanoma cells derived from two different patients were studied using DNA-mediated gene transfer into NIH 3T3 cells followed by tumor induction into athymic nude nice. 64% of the mice injected subcutaneously with selected cells which had been co-transfected with human melanoma DNA and the selective marker NeoR developed tumors within 3-4 weeks, while up to 100% of those injected with cells transfected three days before with melanoma DNA developed tumors within 4-6 weeks. Southern blots analysis of the tumors indicated that almost all of them contained human sequences. Hybridization with different oncogene probes showed the presence of an human Eco RI N-ras-hybridizing fragment in the primary and secondary derived tumors, indicating that a transforming N-ras oncogene in human melanoma had been transferred to recipient cells and that transformed cells induced tumors in nude mice.
利用DNA介导的基因转移技术，将来自两名不同患者的人黑色素瘤细胞导入NIH 3T3细胞，随后将其接种到无胸腺裸鼠体内，研究其致癌潜能。皮下注射经人黑色素瘤DNA和选择标记NeoR共转染的所选细胞的小鼠中，64%在3 - 4周内长出肿瘤，而注射三天前用黑色素瘤DNA转染的细胞的小鼠中，高达100%在4 - 6周内长出肿瘤。对肿瘤的Southern印迹分析表明，几乎所有肿瘤都含有人类序列。与不同癌基因探针杂交显示，在原发和继发肿瘤中存在一个人类Eco RI N-ras杂交片段，表明人黑色素瘤中的一个转化型N-ras癌基因已转移到受体细胞中，且转化细胞在裸鼠体内诱发了肿瘤。
