Department of Chemistry, Graduate School of Science, Nagoya University, Furo-Cho Chikusa-Ku, Nagoya, Aichi, 464-8602, Japan.
Research Center for Materials Science, Nagoya University, Furo-Cho Chikusa-Ku, Nagoya, Aichi, 464-8602, Japan.
Chembiochem. 2018 Aug 6;19(15):1601-1604. doi: 10.1002/cbic.201800256. Epub 2018 Jun 27.
Peptide nucleic acid (PNA) can form a stable duplex with DNA, and, accordingly, directly recognize double-stranded DNA through the formation of a double-duplex invasion complex, wherein a pair of complementary PNA strands form two PNA/DNA duplexes. Because invasion does not require prior denaturation of DNA, PNA holds great potential for in cellulo or in vivo applications. To broaden the applicability of PNA invasion, we developed a new conjugate of PNA with a ruthenium complex. This Ru-PNA conjugate exhibits higher DNA-binding affinity, which results in enhanced invasion efficiency, even under physiological conditions.
肽核酸（PNA）可以与 DNA 形成稳定的双链，并且可以通过形成双链入侵复合物直接识别双链 DNA，其中一对互补的 PNA 链形成两个 PNA/DNA 双链。由于入侵不需要 DNA 预先变性，因此 PNA 在细胞内或体内应用中具有很大的潜力。为了拓宽 PNA 入侵的适用性，我们开发了一种与钌配合物结合的新型 PNA 缀合物。该 Ru-PNA 缀合物表现出更高的 DNA 结合亲和力，这导致即使在生理条件下也能提高入侵效率。
