[CD4⁺CD25⁺调节性T细胞及CCL17、CCL22在头颈部鳞状细胞癌发病机制中的作用]
[The role of CD4 ⁺ CD25 ⁺ T regs and CCL17, CCL22 in the pathogenesis of head and neck squamous cell carcinoma].
作者信息
Gong S C, Zhang H D, Yu Z K, Xing G Q
机构信息
Department of Otolaryngology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
Department of Otolaryngology, Nanjing Tongren Hospital, Affiliated to School of Medicine, Southeast University.
出版信息
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 Oct 20;31(20):1557-1560. doi: 10.13201/j.issn.1001-1781.2017.20.004.
To investigate the role of CD4 ⁺ CD25 ⁺ T regs and CCL17 and CCL22 in the pathogenesis of HNSCC.Twenty cases of HNSCC were enrolled. All patients were primary or recurrent after treatment (chemotherapy, surgery). The primary tumor was taken as the experimental group, and the adjacent normal tissues from the primary tumor 1-3 cm were taken as control group. CD4 ⁺ /Foxp3 and CD25⁺/Foxp3 were detected by immunofluorescence, while CCL17 and CCL22 were detected by ELISA. The difference and correlation between the amount of CD4⁺,CD25⁺ and the expression of CCL17, CCL22 were observed and analyzed.The difference of mean optical density between CD4⁺/Foxp3 and CD25⁺/Foxp3 was statistically significant between the experimental group and the control group (<0.05). The concentration of CCL17 and CCL22 was statistically different between the two groups (<0.01). There was a positive correlation between CD25⁺and CCL17,CCL22(=0.595, 0.720,<0.01).CD4⁺CD25⁺T regs and CCL17,CCL22 played an important role in the pathogenesis of head and neck squamous cell carcinoma,both of which interacted with each other,and promoted the recurrence and metastasis of HNSCC.
探讨CD4⁺CD25⁺调节性T细胞（Tregs）以及CCL17和CCL22在头颈部鳞状细胞癌（HNSCC）发病机制中的作用。纳入20例HNSCC患者。所有患者均为初发或治疗（化疗、手术）后复发。将原发肿瘤作为实验组，取原发肿瘤1 - 3 cm处的相邻正常组织作为对照组。通过免疫荧光检测CD4⁺/Foxp3和CD25⁺/Foxp3，采用酶联免疫吸附测定（ELISA）检测CCL17和CCL22。观察并分析CD4⁺、CD25⁺数量与CCL17、CCL22表达之间的差异及相关性。实验组与对照组之间，CD4⁺/Foxp3和CD25⁺/Foxp3的平均光密度差异具有统计学意义（<0.05）。两组之间CCL17和CCL22的浓度差异具有统计学意义（<0.01）。CD25⁺与CCL17、CCL22之间存在正相关（r = 0.595、0.720，<0.01）。CD4⁺CD25⁺Tregs以及CCL17、CCL22在头颈部鳞状细胞癌发病机制中起重要作用，二者相互作用，促进HNSCC的复发和转移。
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