[鼻息肉中血管内皮生长因子-B甲基化的探究]

[The exploration of VEGF-B methylation in the nasal polyp].

作者信息

Tan H Y, Bai W L, Wang X W, Xu T T

机构信息

Department of Otolaryngology,Shengjing Hospital,the Affiliated Hospital of China Medical University,Shenyang,110004,China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 Oct 5;31(19):1482-1484;1491. doi: 10.13201/j.issn.1001-1781.2017.19.005.

DOI:10.13201/j.issn.1001-1781.2017.19.005

PMID:29798099

Abstract

The objective of this study is to investigate the methylation status of vascular endothelial cell growth factor B (VEGF-B) gene and to indentify the roles in pathogenesis,development and classification of nasal polyps.The methylation status of VEGF-B gene of 28 nasal polyp tissues and 12 samples of inferior turbinate tissues were detected by methylationspecific-polymerase chain reaction (MS-PCR) and gene sequencing.There was significant statistic diference between nasal polyp tissue group and control group (χ ²=4.096,<0.05). The results of gene sequencing suggest that the VEGF-B gene promoter were hypomethylation status in the nasal polyps.Methylation status of VEGF-B promoter may play an important role in the pathogenetic mechanism of nasal polyps.

摘要

本研究的目的是调查血管内皮细胞生长因子B（VEGF-B）基因的甲基化状态，并确定其在鼻息肉发病机制、发展及分类中的作用。采用甲基化特异性聚合酶链反应（MS-PCR）和基因测序检测28例鼻息肉组织及12例下鼻甲组织样本中VEGF-B基因的甲基化状态。鼻息肉组织组与对照组之间存在显著统计学差异（χ²=4.096，P<0.05）。基因测序结果表明，鼻息肉中VEGF-B基因启动子处于低甲基化状态。VEGF-B启动子的甲基化状态可能在鼻息肉的发病机制中起重要作用。