Fox R I, Fong S, Chen P P, Kipps T J
Department of Basic and Clinical Research, Research Institute of Scripps Clinic, La Jolla, California 92037.
In Vivo. 1988 Jan-Feb;2(1):47-55.
Patients with primary Sjögren's syndrome (SS) have rheumatoid factor (anti-IgG Fc antibody, RF) in their sera that contains a crossreactive idiotype (CRI) defined by monoclonal antibody 17-109. This CRI is shared by SS patients and certain patients with Waldenstrom's macroglobulinemia. This CRI was not found in increased frequency in RF from rheumatoid arthritis patients lacking SS. The structural basis for this CRI is the highly conserved kappa chain of the Vk IIIb sub-subgroup. In SS patients, the CRI is present on both IgA RF and IgM RF, accounting for 5-20% of the total RF. Recent studies have shown that a germ line encoded kappa variable region gene (Humkv 325, cloned from a placental DNA library) has DNA sequence that encodes the variable region amino acid sequences of CRI positive kappa chains. Using MoAb 17-109, we have been able to determine the tissue distribution of CRI+ B-cells. We found an increased frequency in the salivary gland biopsies of SS patients and also in their intestinal mucosal tissues (i.e. Peyer's patch). In comparison, we also detected an increased frequency of CRI+ B-cells in the Peyer's patch regions of normals. Taken together, these results suggest that CRI+ B-cells may play an important role in normal mucosal immunity and these CRI+ B-cells have migrated to the salivary gland in SS patients as part of the inflammatory process directed at autoantigens or exogenous antigen(s) located at a mucosal surface.(ABSTRACT TRUNCATED AT 400 WORDS)
原发性干燥综合征（SS）患者血清中含有类风湿因子（抗IgG Fc抗体，RF），该类风湿因子包含由单克隆抗体17 - 109定义的交叉反应独特型（CRI）。SS患者和某些瓦尔登斯特伦巨球蛋白血症患者共享这种CRI。在没有SS的类风湿关节炎患者的RF中，未发现这种CRI频率增加。这种CRI的结构基础是Vk IIIb亚亚组高度保守的κ链。在SS患者中，CRI存在于IgA RF和IgM RF上，占总RF的5% - 20%。最近的研究表明，一个种系编码的κ可变区基因（Humkv 325，从胎盘DNA文库中克隆）具有编码CRI阳性κ链可变区氨基酸序列的DNA序列。使用单克隆抗体17 - 109，我们能够确定CRI + B细胞的组织分布。我们发现SS患者唾液腺活检组织以及肠道黏膜组织（即派尔集合淋巴结）中CRI + B细胞频率增加。相比之下，我们在正常人的派尔集合淋巴结区域也检测到CRI + B细胞频率增加。综上所述，这些结果表明CRI + B细胞可能在正常黏膜免疫中起重要作用，并且在SS患者中，这些CRI + B细胞已迁移至唾液腺，作为针对位于黏膜表面的自身抗原或外源性抗原的炎症过程的一部分。（摘要截选至400字）
