Fox R I, Carson D A, Chen P, Fong S
Scand J Rheumatol Suppl. 1986;61:83-8.
Sjögren's syndrome (SS) is characterized by lymphoid infiltration of the salivary glands and autoantibody production. Rheumatoid factor (RF) in patients with primary SS (1 degree SS) contains a crossreactive idiotype (CRI) defined by a monoclonal antibody (MoAb 17-109). This CRI was located on the kappa light chain by immunoblotting methods. A high frequency of CRI+ B cells was found in SS salivary gland biopsies, suggesting this tissue as the site of production for this autoantibody. Further characterization of CRI+ RF from SS patients was performed using antibodies prepared against synthetic peptides corresponding to the hypervariable region of RF paraproteins from patients with Waldenström's macroglobulinemia (WM). These results demonstrate a close structural relationship between RF in SS and WM patients. To analyze the genes that encode these RF in SS patients, B cell hybridomas that secreted CRI+ immunoglobulin were created and their DNA analyzed by Southern blot techniques. These hybridomas will allow us to determine the DNA sequence of kappa genes encoding the CRI and to identify adjacent regulatory genes that may promote high levels of CRI expression.
干燥综合征（SS）的特征是唾液腺的淋巴细胞浸润和自身抗体产生。原发性干燥综合征（1型SS）患者的类风湿因子（RF）含有一种由单克隆抗体（MoAb 17 - 109）定义的交叉反应独特型（CRI）。通过免疫印迹法确定该CRI位于κ轻链上。在SS唾液腺活检中发现高频率的CRI + B细胞，提示该组织是这种自身抗体的产生部位。使用针对与华氏巨球蛋白血症（WM）患者RF副蛋白高变区对应的合成肽制备的抗体，对SS患者的CRI + RF进行了进一步表征。这些结果表明SS患者和WM患者的RF之间存在密切的结构关系。为了分析SS患者中编码这些RF的基因，创建了分泌CRI +免疫球蛋白的B细胞杂交瘤，并通过Southern印迹技术分析其DNA。这些杂交瘤将使我们能够确定编码CRI的κ基因的DNA序列，并鉴定可能促进CRI高水平表达的相邻调控基因。
