Li S Q, Liu P F, Cui P C
Department of Otolaryngology Head and Neck Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Mar;32(5):360-364. doi: 10.13201/j.issn.1001-1781.2018.05.010.
To prepare and to compare the decellularized rat cricoarytenoid dorsalis muscle and gastrocnemius muscle scaffolds for providing a basis for tissue engineered cricoarytenoid dorsalis muscle.Sprague Dawley rats were randomly divided into 6 groups with 10 rats in each group.The experimental groups were divided as 2, 4, 6, 8, and 10day groups after decellularization. Muscles were decellularized with 1% sodium dodecyl sulfate and observed by general observation, histological section with hematoxylin eosin staining and collagenous fiber staining, scanning electron microscope images with measurement of pore diameter and area, and DNA extraction and quantification. The control group was not decellularized.The decellularized muscles in experimental groups showed a semitransparent appearance while in control group the fresh muscle showed a ruddy color.Muscle fibers were completely disappeared in cricoarytenoid dorsalis muscle after 4 days decellularization and in gastrocnemius muscle after 8 days decellularzation, the extracellular matrix reserved well and no DNA was detected. Scanning electron microscope revealed a different pore size between decellularized cricoarytenoid dorsalis muscle and gastrocnemius muscle,and the difference was statistically significant. The pore diameter in 4 days decellularized cricoarytenoid dorsalis muscle was larger than the one in 8 days decellularzed gastrocnemius muscle.Compare to gastrocnemius muscle,decellularized cricoarytenoid dorsalis muscle has advantages in pore form and size and more suitable as a scaffold for tissue engineered cricoarytenoid dorsalis muscle.
制备并比较去细胞大鼠环杓后肌和腓肠肌支架，为组织工程化环杓后肌提供基础。将Sprague Dawley大鼠随机分为6组，每组10只。实验组分为去细胞后2、4、6、8和10天组。用1%十二烷基硫酸钠对肌肉进行去细胞处理，并通过大体观察、苏木精-伊红染色和胶原纤维染色的组织学切片、扫描电子显微镜图像（测量孔径和面积）以及DNA提取和定量分析进行观察。对照组不进行去细胞处理。实验组去细胞后的肌肉呈现半透明外观，而对照组新鲜肌肉呈红润颜色。环杓后肌去细胞4天后肌纤维完全消失，腓肠肌去细胞8天后肌纤维完全消失，细胞外基质保留良好且未检测到DNA。扫描电子显微镜显示去细胞的环杓后肌和腓肠肌的孔径不同，差异具有统计学意义。去细胞4天的环杓后肌的孔径大于去细胞8天的腓肠肌的孔径。与腓肠肌相比，去细胞的环杓后肌在孔的形态和大小方面具有优势，更适合作为组织工程化环杓后肌的支架。
