[Preparation and comparative study of decellularized rat cricoarytenoid dorsalis muscle and gastrocnemius muscle scaffold].

To prepare and to compare the decellularized rat cricoarytenoid dorsalis muscle and gastrocnemius muscle scaffolds for providing a basis for tissue engineered cricoarytenoid dorsalis muscle.Sprague Dawley rats were randomly divided into 6 groups with 10 rats in each group.The experimental groups were divided as 2, 4, 6, 8, and 10day groups after decellularization. Muscles were decellularized with 1% sodium dodecyl sulfate and observed by general observation, histological section with hematoxylin eosin staining and collagenous fiber staining, scanning electron microscope images with measurement of pore diameter and area, and DNA extraction and quantification. The control group was not decellularized.The decellularized muscles in experimental groups showed a semitransparent appearance while in control group the fresh muscle showed a ruddy color.Muscle fibers were completely disappeared in cricoarytenoid dorsalis muscle after 4 days decellularization and in gastrocnemius muscle after 8 days decellularzation, the extracellular matrix reserved well and no DNA was detected. Scanning electron microscope revealed a different pore size between decellularized cricoarytenoid dorsalis muscle and gastrocnemius muscle,and the difference was statistically significant. The pore diameter in 4 days decellularized cricoarytenoid dorsalis muscle was larger than the one in 8 days decellularzed gastrocnemius muscle.Compare to gastrocnemius muscle,decellularized cricoarytenoid dorsalis muscle has advantages in pore form and size and more suitable as a scaffold for tissue engineered cricoarytenoid dorsalis muscle.