Ren H L, Li J D, Miao Y H, Xu T
Department of Otolaryngology, Beijing Electric Power Hospital, State Grid Corporation of China, Beijing, 100073, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Mar;32(5):365-368. doi: 10.13201/j.issn.1001-1781.2018.05.011.
To investigate the clinical application of glass micro fiber basophil activation test (BAT) used as a complementary test for house dust mite allergen.Forty patients with clinical diagnosed allergic rhinitis was test by three methods for house dust mite allergen, skin prick test(SPT),Immuno CAP sIgE, and BAT in vitro. The sensitivity and specificity of glass micro fiber were accessed, and the consistency between BAT, SPT, and Immuno sIgE was analyzed. As in vivo provocation was not performed, gold standard is regarded as the combination of medical history and positive reports of SPT and/or ImmunoCAP sIgE test.Twentythree patients are diagnosed as house dust mite allergic rhinitis by gold standard. The sensitivity and specificity of glass micro fiber BAT were 60.9% and 88.2%, the sensitivity of SPT and sIgE was 87.0% and sIgE 73.9%. The correlation rates between BAT with SPT is 0.67(<0.05), and sIgE 0.55(<0.05). The accuracy, predictive value of positive and negative of BAT are 0.47,60.9%,88.2%.The Kappa values of BAT, SPT and sIgE with gold standard are 0.47,0.86,0.71.As a complementary test for house dust mite allergic rhinitis, BAT have a good consistency with SPT and sIgE, while as it has only moderate consistency with "gold standard", further studies are needed to prove its clinical significance.
探讨玻璃微纤维嗜碱性粒细胞活化试验（BAT）作为屋尘螨过敏原补充试验的临床应用。对40例临床诊断为变应性鼻炎的患者采用屋尘螨过敏原的三种检测方法，即皮肤点刺试验（SPT）、免疫CAP sIgE和体外BAT进行检测。评估玻璃微纤维的敏感性和特异性，并分析BAT、SPT和免疫sIgE之间的一致性。由于未进行体内激发试验，将病史以及SPT和/或免疫CAP sIgE试验的阳性报告相结合视为金标准。23例患者经金标准诊断为屋尘螨变应性鼻炎。玻璃微纤维BAT的敏感性和特异性分别为60.9%和88.2%，SPT和sIgE的敏感性分别为87.0%和73.9%。BAT与SPT的相关率为0.67（<0.05），与sIgE的相关率为0.55（<0.05）。BAT的准确性、阳性预测值和阴性预测值分别为0.47、60.9%、88.2%。BAT、SPT和sIgE与金标准的Kappa值分别为0.47、0.86、0.71。作为屋尘螨变应性鼻炎的补充试验，BAT与SPT和sIgE具有良好的一致性，而与“金标准”仅具有中等一致性，需要进一步研究以证明其临床意义。
