成熟前培养时间对牛小型卵母细胞发育能力的影响。
Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes.
作者信息
Abdel-Ghani Mohammed A, Sakaguchi Kenichiro, Kanno Chihiro, Yanagawa Yojiro, Katagiri Seiji, Nagano Masashi
机构信息
Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Department of Theriogenology, Faculty of Veterinary Medicine, Assuit University, Assuit, 71515, Egypt.
出版信息
J Reprod Dev. 2018 Aug 20;64(4):365-369. doi: 10.1262/jrd.2018-004. Epub 2018 May 24.
We investigated the effects of pre-maturational (pre-IVM) culture on the developmental competence of small-sized bovine oocytes (110 and < 115 µm). Oocytes were cultured with 3-isobutyl-1-methylxanthine (IBMX) for 0, 5, or 10 h and subjected to in vitro maturation, fertilization, and culture. The cleavage rate (73%) of small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (61 and 62%, respectively). The blastocyst rate (16%) of embryos derived from small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (9 and 8%, respectively). In addition, small-sized oocytes with 5 h pre-IVM had a higher mean cell number in blastocysts (134.1 ± 34.8) than those with 0 and 10 h pre-IVM (100.2 ± 17.2 and 107.8 ± 23.7, respectively). In conclusion, the pre-IVM of small-sized oocytes with IBMX for 5 h improved the developmental competence of bovine oocytes, as well as the quality of blastocysts.
我们研究了成熟前（IVM前）培养对小型牛卵母细胞（直径110和<115 µm）发育能力的影响。将卵母细胞与3-异丁基-1-甲基黄嘌呤（IBMX）培养0、5或10小时，然后进行体外成熟、受精和培养。IVM前培养5小时的小型卵母细胞的卵裂率（73%）高于IVM前培养0小时和10小时的卵母细胞（分别为61%和62%）。IVM前培养5小时的小型卵母细胞来源胚胎的囊胚率（16%）高于IVM前培养0小时和10小时的胚胎（分别为9%和8%）。此外，IVM前培养5小时的小型卵母细胞形成的囊胚平均细胞数（134.1±34.8）高于IVM前培养0小时和10小时的卵母细胞（分别为100.2±17.2和107.8±23.7）。总之，用IBMX对小型卵母细胞进行5小时的IVM前培养可提高牛卵母细胞的发育能力以及囊胚质量。
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