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早期培养促进了源自 8 天体外生长培养系统的牛卵母细胞的发育能力。

Pre-maturational culture promotes the developmental competence of bovine oocytes derived from an 8-day in vitro growth culture system.

机构信息

Laboratory of Theriogenology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.

Department of Clinical Studies, Faculty of Veterinary Medicine, Lilongwe University of Agriculture and Natural Resources, Malawi.

出版信息

J Reprod Dev. 2023 Aug 11;69(4):214-217. doi: 10.1262/jrd.2023-022. Epub 2023 May 18.

DOI:10.1262/jrd.2023-022
PMID:37197977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10435529/
Abstract

In this study, we evaluated the effects of pre-maturational culture (pre-IVM) on the developmental competence of bovine oocytes derived from an 8-day in vitro growth (IVG) culture system. IVG oocytes were subjected to 5 h pre-IVM before in vitro maturation, followed by in vitro fertilization (IVF). The proportion of oocytes that progressed to the germinal vesicle breakdown stage was similar between groups with and without pre-IVM. Although metaphase II oocytes and cleavage rates after IVF were similar regardless of pre-IVM culture, the blastocyst rate was significantly higher in the group with pre-IVM (22.5%) than without pre-IVM (11.0%, P < 0.05). In conclusion, pre-IVM culture improved the developmental competence of bovine oocytes derived from an 8-day IVG system.

摘要

在这项研究中，我们评估了预体外成熟培养（pre-IVM）对源自 8 天体外生长（IVG）培养系统的牛卵母细胞发育能力的影响。在体外成熟之前，将 IVG 卵母细胞进行 5 小时的 pre-IVM 处理，然后进行体外受精（IVF）。有和没有 pre-IVM 的组之间，卵母细胞进展到生发泡破裂阶段的比例相似。尽管无论是否进行 pre-IVM 培养，中期 II 卵母细胞和 IVF 后的卵裂率相似，但具有 pre-IVM 的组的囊胚率明显更高（22.5%比没有 pre-IVM 的组 11.0%，P<0.05）。总之，pre-IVM 培养提高了源自 8 天 IVG 系统的牛卵母细胞的发育能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d097/10435529/262fb0123167/jrd-69-214-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d097/10435529/262fb0123167/jrd-69-214-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d097/10435529/262fb0123167/jrd-69-214-g001.jpg

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本文引用的文献

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Theriogenology. 2022 Jan 1;177:116-126. doi: 10.1016/j.theriogenology.2021.10.014. Epub 2021 Oct 18.
2
Approaches to oocyte meiotic arrest in vitro and impact on oocyte developmental competence.体外卵母细胞减数分裂阻滞方法及其对卵母细胞发育能力的影响。
Biol Reprod. 2022 Feb 22;106(2):243-252. doi: 10.1093/biolre/ioab176.
3
Effect of increased oxygen availability and astaxanthin supplementation on the growth, maturation and developmental competence of bovine oocytes derived from early antral follicles.
增加氧气供应和补充虾青素对源自早期窦卵泡的牛卵母细胞生长、成熟和发育能力的影响。
Theriogenology. 2020 Nov;157:341-349. doi: 10.1016/j.theriogenology.2020.07.023. Epub 2020 Aug 10.
4
Bovine oocyte maturation: acquisition of developmental competence.牛卵母细胞成熟：发育能力的获得。
Reprod Fertil Dev. 2019 Jan;32(2):98-103. doi: 10.1071/RD19255.
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Theriogenology. 2013 Oct 15;80(7):793-9. doi: 10.1016/j.theriogenology.2013.07.004. Epub 2013 Aug 15.