Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China.
Faculty of Chemistry, Northeast Normal University, Changchun 130024, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Sep 5;202:305-313. doi: 10.1016/j.saa.2018.05.045. Epub 2018 May 26.
A new benzimidazole base turn-on fluorescent and ratiometric absorption chemosensor (L) bearing bidentate ligand for detection of Cu was designed and synthesized. Fluorescence and UV-vis spectra studies demonstrated that L can detect Cu ions in aqueous solution using fluorescence enhancement and ratiometric absorption sensing over a wide pH range. Both fluorescent and ratiometric absorption sensing of L for Cu possessed high selectivity and sensitivity over other competitive metal ions and had low detection limit. Job's plot, mass spectra and DFT calculation indicated the sensing mechanism is the complex formation between L and Cu with 1:2 stoichiometry. Fluorescence images of HepG2 in the absence and presence of Cu displayed L had cell permeability and detection ability for Cu in live cells.
一种新型的苯并咪唑基双齿配体开环荧光和比率吸收型化学传感器（L）被设计并合成出来，用于检测 Cu。荧光和紫外可见光谱研究表明，L 可以在宽 pH 范围内通过荧光增强和比率吸收传感来检测水溶液中的 Cu 离子。L 对 Cu 的荧光和比率吸收传感具有高选择性和灵敏度，超过其他竞争金属离子，并且具有低检测限。Job 图、质谱和 DFT 计算表明，传感机制是 L 与 Cu 形成 1:2 化学计量比的配合物。在没有和存在 Cu 的情况下，HepG2 的荧光图像显示 L 具有细胞通透性，并能够在活细胞中检测 Cu。
