Department of Chemistry, Faculty of Sciences and Arts, Bingol University, Bingol, Turkey.
Department of Chemistry, Faculty of Sciences and Arts, Bingol University, Bingol, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 15;213:6-11. doi: 10.1016/j.saa.2019.01.053. Epub 2019 Jan 16.
Detection of copper attracts important in most environmental and biological systems. In this study, a simple probe BisPyTSC containing bis-pyrene core was synthesized, and cation binding and sensing properties were studied using colorimetric and fluorometric detection. The research indicated that the specific ligand affinity for Cu ions results in drastic color and spectral changes. According to the data obtained, while the peak intensity increases at 376 nm, the peak intensity decreased at 280 nm in the absorption spectrum of BisPyTSC and an increase in fluorescence intensity of BisPyTSC was observed in the presence of Cu ions. The binding ratio of BisPyTSC to Cu was found to be 1:1 according to Job's plot experiments. The binding constant was calculated using the Benesi-Hildebrand equation and found to be 3.26 × 10 M. Based on these concentration dependent fluorescence changes, the limit of detection (LOD) value was calculated to be 14.5 μM for Cu, which is the range of copper that should be in the blood (11.8-23.6 μM). As a result of all these studies, we can understand that BisPyTSC is a good selective candidate turn-on sensor that can be used for Cu detection.
铜的检测在大多数环境和生物系统中都很重要。在这项研究中，合成了一种含有双芘核心的简单探针 BisPyTSC，并通过比色法和荧光法检测研究了其阳离子结合和传感性能。研究表明，特定配体对 Cu 离子的亲和力导致了剧烈的颜色和光谱变化。根据获得的数据，当 BisPyTSC 的吸收光谱中 376nm 处的峰强度增加时，280nm 处的峰强度降低，并且在存在 Cu 离子时观察到 BisPyTSC 的荧光强度增加。根据 Job 图实验，发现 BisPyTSC 与 Cu 的结合比为 1:1。根据 Benesi-Hildebrand 方程计算了结合常数，发现其值为 3.26×10 M。基于这些浓度依赖性荧光变化，计算出 Cu 的检测限（LOD）值为 14.5μM，这是血液中铜的含量范围（11.8-23.6μM）。通过所有这些研究，我们可以理解 BisPyTSC 是一种良好的选择性荧光开启型传感器，可用于 Cu 的检测。
