Kitagawa T, Ono K
Department of Anatomy, Asahikawa Medical College, Japan.
Histol Histopathol. 1986 Jan;1(1):49-57.
Ultrastructural changes of the pancreatic exocrine cells after 3, 7, 14, 21, 28, 35 and 42 days of starvation were observed in male rats aged from 16 to 18 months weighing between 600 and 700 grams. The number of zymogen granules after starvation decreased to less than about 70 per cent of that of the control. Changes in the rough endoplasmic reticulum were hardly seen up to 14 days of starvation as compared with the control, but were observed in the apical and basal cytoplasm of the cell from 21 days after starvation. Particularly in 35- and 42-day starved rats, the rough endoplasmic reticulum was frequently shortened and dilated, and changed to disorganized membranous structures. The lysosomes in the apical cytoplasm of the cell gradually increased in number after starvation, and contact or fusion between the zymogen granules and lysosomes (viz, so-called crinophagy) was often seen at 35 and 42 days of starvation. Large autolysosomes especially those containing zymogen granules and rough endoplasmic reticulum were also marked in the basal cytoplasm of the cell after 35 and 42 days of starvation. Alterations in the basal cytoplasm of the cell appeared later than those in the apical cytoplasm. It was considered that, owing to its role in protein synthesis, the basal cytoplasm of the pancreatic exocrine cells in starved rats might be protected as far as possible during long-term starvation.
观察了体重在600至700克之间、年龄在16至18个月的雄性大鼠在饥饿3、7、14、21、28、35和42天后胰腺外分泌细胞的超微结构变化。饥饿后酶原颗粒数量减少至对照组的约70%以下。与对照组相比，饥饿14天内粗面内质网几乎未见变化，但饥饿21天后在细胞的顶端和基底细胞质中观察到变化。特别是在饥饿35天和42天的大鼠中，粗面内质网经常缩短和扩张，并转变为杂乱的膜结构。饥饿后细胞顶端细胞质中的溶酶体数量逐渐增加，在饥饿35天和42天时，经常可见酶原颗粒与溶酶体之间的接触或融合（即所谓的自噬性溶酶体形成）。在饥饿35天和42天后，细胞基底细胞质中也可见大量自噬溶酶体，尤其是含有酶原颗粒和粗面内质网的自噬溶酶体。细胞基底细胞质的变化比顶端细胞质的变化出现得晚。据认为，由于其在蛋白质合成中的作用，饥饿大鼠胰腺外分泌细胞的基底细胞质在长期饥饿期间可能会尽可能得到保护。
