Roos N
Electron Microscopical Unit for Biological Sciences, University of Oslo, Norway.
Scanning Microsc. 1988 Mar;2(1):323-9.
We analysed four subcellular compartments in rat exocrine pancreas cells, zymogen granules, cytoplasm surrounding the zymogen granules, mitochondria and cytoplasm in the basal part of the cells for sodium, magnesium, phosphorus, sulfur, chlorine, potassium and calcium content, using ultrathin frozen-dried cryosections. The highest concentrations of calcium were measured in the zymogen granules and the surrounding apical part of the cell containing Golgi apparatus, smooth endoplasmic reticulum and condensing vacuoles. Calcium concentrations in the basal part of the cells (mostly rough endoplasmic reticulum) were 60% lower than in the apical part of the cells. The lowest calcium concentrations were measured in mitochondria. The results suggest that other subcellular compartments than the rough endoplasmic reticulum and mitochondria might be involved in the intracellular Ca2+ regulation.
我们使用超薄冷冻干燥低温切片，分析了大鼠外分泌胰腺细胞中的四个亚细胞区室，即酶原颗粒、酶原颗粒周围的细胞质、线粒体以及细胞基部的细胞质中的钠、镁、磷、硫、氯、钾和钙含量。在酶原颗粒以及含有高尔基体、光滑内质网和浓缩泡的细胞顶端周围部分测得的钙浓度最高。细胞基部（主要是粗面内质网）的钙浓度比细胞顶端部分低60%。线粒体中的钙浓度最低。结果表明，除粗面内质网和线粒体外，其他亚细胞区室可能参与细胞内Ca2+的调节。
