Weischer C H, Krisinger J, Karzel K
Institut für Pharmakologie und Toxikologie, Universität Bonn, FRG.
Histol Histopathol. 1986 Jul;1(3):303-8.
The aim of this study was to develop a standardized image analysis method for localization and quantitative measurement of calcified structures of murine fetal tibiae cultured in vitro as a completion and verification of previous biochemical studies. The calcified structures of bone stained by von Kossa silver technique and the epiphyseal cartilages showing intensive metachromasia with toluidine-blue staining were converted with grey-value window programs and afterwards the areas of the selected structures were measured. The histomorphological investigations showed that the murine tibiae, incubated for a period of 6 days in a medium with addition of 5 mmol mellitic acid, showed both a significant reduction of calcium deposits and an increase of epiphyseal intercellular cartilage matrix. The tibiae incubated in a medium with addition of 0.5 mmol sodium fluoride significantly showed an increase of calcium deposits in the thickened lamellae of the compacta. These histomorphological results confirm previous biochemical studies.
本研究的目的是开发一种标准化的图像分析方法，用于体外培养的小鼠胎儿胫骨钙化结构的定位和定量测量，作为对先前生化研究的补充和验证。用冯·科萨银染技术染色的骨钙化结构和经甲苯胺蓝染色显示强异染性的骨骺软骨，通过灰度值窗口程序进行转换，然后测量所选结构的面积。组织形态学研究表明，在添加5 mmol苯六甲酸的培养基中孵育6天的小鼠胫骨，钙沉积显著减少，骨骺细胞间软骨基质增加。在添加0.5 mmol氟化钠的培养基中孵育的胫骨，在致密骨增厚的板层中钙沉积显著增加。这些组织形态学结果证实了先前的生化研究。
