Weischer C H, Frey K D, Karzel K
Arzneimittelforschung. 1980;30(2):251-4.
The histological and histochemical appearance of murine fetal tibiae was studied in order to furnish the morphological background for biochemical investigations on in vitro cultures of these organs, used as an experimental model for the study of drug influences on metabolic processes of mesenchymal tissues. Freshly excised tibiae until the 14th day of gestation showed solely cartilagenous structures; first signs of calcification were found after 15 days of gestation and ossification of the diaphysis proceeds until the 17th day. A comparison of tibiae explants excised on the 17th day of gestation and cultured for 6 days in vitro with freshly excised tibiae of the same age (17 days) indicates a continuous growth of the explants in vitro without any signs of degenerative processes which could be due to the culture conditions.
为了为这些器官的体外培养的生化研究提供形态学背景，研究了小鼠胎儿胫骨的组织学和组织化学外观，这些器官的体外培养用作研究药物对间充质组织代谢过程影响的实验模型。妊娠第14天之前新鲜切除的胫骨仅显示软骨结构；妊娠15天后发现钙化的最初迹象，骨干的骨化持续到第17天。将妊娠第17天切除并在体外培养6天的胫骨外植体与相同年龄（17天）的新鲜切除的胫骨进行比较，结果表明外植体在体外持续生长，没有任何可能归因于培养条件的退化过程迹象。
