Palukaitis P
Department of Plant Pathology, Cornell University, Ithaca, NY 14853.
Mol Plant Microbe Interact. 1988 Apr;1(4):175-81. doi: 10.1094/mpmi-1-175.
Six satellite RNAs of cucumber mosaic virus (CMV) could be differentiated on the basis of symptom expression they elicit in tomato and tobacco, and all but two could be differentiated by gel electrophoretic migration. Three of the satellite RNAs (B2-sat, G-sat, and WL1-sat RNA) ameliorated the symptoms induced by CMV on tomato, whereas three others (B1-sat, B3-sat, and WL2-sat RNA) induced chlorosis on tomato, the extent and nature of which was CMV-strain dependent. By contrast, B2-sat RNA induced chlorosis in tobacco, whereas WL1-sat and G-sat RNAs did not. Thus, the symptoms observed were dependent on the host species, the particular satellite RNA, and also the strain of helper virus, suggesting that a complex association of at least three factors is involved in symptom elicitation. Comparisons of the nucleotide sequences of pairs of satellite RNAs inducing the various chlorotic responses suggest that only a few nucleotide changes in specific domains are required for the elicitation of different host responses.
黄瓜花叶病毒（CMV）的六种卫星RNA可根据它们在番茄和烟草中引发的症状表现来区分，除了两种之外，其他所有卫星RNA都可以通过凝胶电泳迁移来区分。三种卫星RNA（B2-sat、G-sat和WL1-sat RNA）减轻了CMV在番茄上诱导的症状，而另外三种（B1-sat、B3-sat和WL2-sat RNA）在番茄上诱导了黄化，其程度和性质取决于CMV毒株。相比之下，B2-sat RNA在烟草中诱导黄化，而WL1-sat和G-sat RNA则不会。因此，观察到的症状取决于宿主物种、特定的卫星RNA以及辅助病毒的毒株，这表明至少三种因素的复杂关联参与了症状引发。对诱导各种黄化反应的卫星RNA对的核苷酸序列进行比较表明，在特定结构域中只需少数核苷酸变化就能引发不同的宿主反应。
