Department of Surgery, University of Michigan School of Medicine, Ann Arbor, MI 48109.
Graduate Program in Immunology, University of Michigan School of Medicine, Ann Arbor, MI 48109.
J Immunol. 2018 Jul 15;201(2):814-820. doi: 10.4049/jimmunol.1700755. Epub 2018 May 25.
Naive T cells are thought to be functionally quiescent. In this study, we studied and compared the phenotype, cytokine profile, and potential function of human naive CD4 T cells in umbilical cord and peripheral blood. We found that naive CD4 T cells, but not memory T cells, expressed high levels of chemokine CXCL8. CXCL8 naive T cells were preferentially enriched CD31 T cells and did not express T cell activation markers or typical Th effector cytokines, including IFN-γ, IL-4, IL-17, and IL-22. In addition, upon activation, naive T cells retained high levels of CXCL8 expression. Furthermore, we showed that naive T cell-derived CXCL8 mediated neutrophil migration in the in vitro migration assay, supported tumor sphere formation, and promoted tumor growth in an in vivo human xenograft model. Thus, human naive T cells are phenotypically and functionally heterogeneous and can carry out active functions in immune responses.
幼稚 T 细胞被认为是功能静止的。在这项研究中，我们研究并比较了脐带血和外周血中人类幼稚 CD4 T 细胞的表型、细胞因子谱和潜在功能。我们发现，幼稚 CD4 T 细胞而非记忆 T 细胞表达高水平的趋化因子 CXCL8。幼稚 T 细胞优先富集 CD31+T 细胞，不表达 T 细胞活化标志物或典型 Th 效应细胞因子，包括 IFN-γ、IL-4、IL-17 和 IL-22。此外，在激活后，幼稚 T 细胞仍保持高水平的 CXCL8 表达。此外，我们表明，幼稚 T 细胞衍生的 CXCL8 在体外迁移实验中介导中性粒细胞迁移，支持肿瘤球体形成，并在体内人异种移植模型中促进肿瘤生长。因此，人类幼稚 T 细胞在表型和功能上具有异质性，可在免疫反应中发挥积极作用。
