Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Sci Rep. 2018 May 25;8(1):8160. doi: 10.1038/s41598-018-26513-2.
Blueberry juice is a healthy and nutritious food that has become increasingly popular worldwide. However, little is known about the microbial groups of this juice that can cause its spoilage. This study aimed to identify the main spoilage microorganisms in blueberry juice and explore whether a microchip pulsed electric field (MPEF) can effectively inactivate them. We performed polymerase chain reaction (PCR) amplification, as well as 16S rDNA, 18S rDNA, internal transcribed spacer (ITS), and 26S rDNA gene sequence analyses. Nine species belonging to eight genera, including Pantoea, Burkholderia, Pichia, Meyerozyma, Cryptococcus, Aureobasidium, Cladosporium, and Penicillium were identified as spoilage microorganisms. Cryptococcus sp., Meyerozyma sp., and Pichia sp. were specific spoilage organisms (SSO) owing to their rising numbers throughout spoilage progression. The effect of MPEF on the potential inactivation of these microorganisms was to induce significant inactivation of viable Cryptococcus sp., Meyerozyma sp., and Pichia sp. This research provides a theoretical basis for the application of MPEF in improving the quality of blueberry juice.
蓝莓汁是一种健康且营养丰富的食品，在全球范围内越来越受欢迎。然而，对于可能导致其变质的蓝莓汁中的微生物群，人们知之甚少。本研究旨在鉴定蓝莓汁中的主要变质微生物，并探讨微芯片脉冲电场（MPEF）是否能有效灭活它们。我们进行了聚合酶链反应（PCR）扩增以及 16S rDNA、18S rDNA、内部转录间隔区（ITS）和 26S rDNA 基因序列分析。共鉴定出 9 个种，属于 8 个属，包括 Pantoea、Burkholderia、Pichia、Meyerozyma、Cryptococcus、Aureobasidium、Cladosporium 和 Penicillium，它们被认为是变质微生物。由于在变质过程中数量不断增加，Cryptococcus sp.、Meyerozyma sp. 和 Pichia sp. 被认为是特定的变质菌（SSO）。MPEF 对这些微生物潜在灭活的效果是诱导活的 Cryptococcus sp.、Meyerozyma sp. 和 Pichia sp. 的显著灭活。本研究为 MPEF 在提高蓝莓汁质量方面的应用提供了理论依据。
