Ottestad L O, Tveit K M, Nesland J M, Dybsjord I, Børmer O, Høie J, Pihl A
Department of Biochemistry, Norwegian Radium Hospital, Oslo.
Histol Histopathol. 1988 Jul;3(3):269-74.
The presence of oestrogen receptors was studied in 105 human breast carcinomas using monoclonal antibodies (Abbott ER-ICA kit). The oestrogen receptors of neoplastic cells were semiquantitatively measured and correlations were made to receptor values determined by a dextran-coated charcoal (DCC) steroid binding assay and to histological grade. Immunoreactive cells were found in about 2/3 of the tumours. Usually only a fraction of the cells within each tumour were immunoreactive, and the staining intensity varied among different cells. In general, well differentiated tumours had a greater proportion of immunoreactive cells than poorly differentiated ones. In most cases (65/98) a good agreement was found between the ER-ICA method and the DCC assay. However, in 33 cases discrepancies were demonstrated.
使用单克隆抗体（雅培ER-ICA试剂盒）对105例人类乳腺癌进行了雌激素受体检测。对肿瘤细胞的雌激素受体进行了半定量测定，并与用葡聚糖包被活性炭（DCC）类固醇结合分析法测定的受体值以及组织学分级进行了相关性分析。在约2/3的肿瘤中发现了免疫反应性细胞。通常每个肿瘤中只有一部分细胞具有免疫反应性，且不同细胞的染色强度有所不同。一般来说，高分化肿瘤中具有免疫反应性的细胞比例高于低分化肿瘤。在大多数情况下（65/98），ER-ICA法与DCC分析法结果吻合良好。然而，有33例出现了差异。
