González J L, Rollán E, Novoa C, Castaño M
Department of Animal Pathology II (Histology and Pathology), Veterinary Faculty of the Complutense University of Madrid, Spain.
Histol Histopathol. 1988 Oct;3(4):323-9.
Six 4 month-old beagles were inoculated with Leishmania donovani infantum, three of them intraperitoneally (Group A) and the other three intravenously (Group B). The animals from Group A were killed 109, 433 and 592 days after inoculation and animals from Group B 109, 171 and 334 after inoculation. The liver of each of them was examined by means of light and electron microscopy. The lesions observed in both groups were very similar, but developed more rapidly in Group B. A chronic hepatitis appeared due to infection, characterized by the presence of multiple intralobular granulomas and portal inflammatory infiltrates consisting of lymphocytes, plasmocytes and macrophages with a variable number of amastigotes. The Kupffer cells were hyperplastic and contained parasites in their cytoplasm. Gradually the hepatocytes developed a progressive cellular swelling, which during the end-stages of the process showed itself with severe nuclear degeneration, disintegration of cytoplasmic organelles, enlargement of the cytoplasmic matrix and disruption of the plasma membranes, leading to cytolysis.
六只4个月大的比格犬接种了婴儿利什曼原虫，其中三只通过腹腔注射（A组），另外三只通过静脉注射（B组）。A组动物在接种后109天、433天和592天处死，B组动物在接种后109天、171天和334天处死。对每只动物的肝脏进行了光镜和电镜检查。两组观察到的病变非常相似，但B组发展得更快。感染导致慢性肝炎，其特征是存在多个小叶内肉芽肿和门脉炎症浸润，浸润细胞包括淋巴细胞、浆细胞和巨噬细胞，还有数量不等的无鞭毛体。库普弗细胞增生，其细胞质中含有寄生虫。肝细胞逐渐出现进行性细胞肿胀，在病程末期表现为严重的核变性、细胞质细胞器解体、细胞质基质增大和质膜破裂，导致细胞溶解。
