Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of Korea.
Division of Biotechnology, Korea University, Seoul, Republic of Korea.
J Sci Food Agric. 2019 Jan 15;99(1):55-63. doi: 10.1002/jsfa.9141. Epub 2018 Jul 3.
Wheat grain is recognized as a rich source of nutrients, including proteins, vitamins, minerals, fibers and antioxidants. In recent years, the focus of wheat breeding has been to increase the content of bioactive compounds to improve human health and prevent diseases.
Five novel wheat mutant lines with variable seed color were developed using gamma irradiation of hexaploid wheat inbred line K4191 (purple seed color). The total anthocyanin contents of three mutant lines (L47, L167 and L925) were significantly higher than those of wild-type lines, including K4191 and 'Keumkang' (white seed color). L925 showed the highest total anthocyanin content, and cyanidin-3-glucoside was presented as the most predominant anthocyanin. Compared with 'Keumkang', the expression of anthocyanin biosynthesis genes was significantly up-regulated in purple seed mutant lines. The highest antioxidant activity was observed in L925 extracts. The expression of a few antioxidant-related genes and total anthocyanin content were positively correlated with antioxidant capacity. These data suggest that anthocyanins and phenolic compounds in wheat grains contribute to the antioxidant potential.
Purple grain color is associated with higher anthocyanin accumulation and antioxidant capacity in wheat. Wheat mutants developed in this study may serve as a valuable source of antioxidants. © 2018 Society of Chemical Industry.
小麦籽粒被认为是营养丰富的来源，包括蛋白质、维生素、矿物质、纤维和抗氧化剂。近年来，小麦育种的重点是提高生物活性化合物的含量，以改善人类健康和预防疾病。
利用六倍体小麦自交系 K4191（紫色种子颜色）的γ射线辐照，开发了五个具有不同种子颜色的新型小麦突变体系。三个突变体系（L47、L167 和 L925）的总花青素含量明显高于野生型系，包括 K4191 和‘Keumkang’（白色种子颜色）。L925 表现出最高的总花青素含量，且以矢车菊素-3-葡萄糖苷为主要花青素。与‘Keumkang’相比，紫色种子突变体系中花青素生物合成基因的表达显著上调。L925 提取物具有最高的抗氧化活性。一些抗氧化相关基因的表达和总花青素含量与抗氧化能力呈正相关。这些数据表明，小麦籽粒中的花青素和酚类化合物有助于其具有抗氧化潜力。
紫色籽粒颜色与小麦中较高的花青素积累和抗氧化能力有关。本研究开发的小麦突变体可能是抗氧化剂的一个有价值的来源。© 2018 英国化学学会。
