Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Food Res Int. 2018 Jul;109:187-195. doi: 10.1016/j.foodres.2018.04.038. Epub 2018 Apr 19.
Taihe black-boned silky fowl (Gallus gallus domesticus Brisson) has a history of over 2200 years of being consumed as a curative food in China. In this work, an LC/MS-based lipidomics approach was employed to investigate the characteristic lipid composition of Taihe black-boned silky fowls from different ages and genders as well as from different carcass parts. Data were processed using an orthogonal partial least squares discriminant analysis and one-way analysis of variance. A total of 1127 lipids were detected in Taihe black-boned silky fowl muscles. Among them, 88, 11 and 1 lipid species were found to have both a variable influence on a projection value >1 and a p-value smaller than 0.05 between different age, gender and part groups. These results illustrate that the influence of the 3 investigated factors on the lipid profiles of Taihe black-boned silky fowl decreased in the order of age > gender > part. Lipid profile differences will facilitate a better understanding of the curative properties of Taihe black-boned silky fowl. Taihe and crossbred black-boned silky fowls were compared in terms of their lipid compositions based on the same strategy. The results showed that the two groups were able to discriminate from each other effectively. 47 lipid compounds were determined to be potential markers for the authentication of Taihe black-boned silky fowl. This work demonstrates the successful application of lipidomics for lipid profiling in food raw materials.
泰和乌鸡（Gallus gallus domesticus Brisson）作为一种具有 2200 多年食用和药用历史的中国传统家禽，其不同年龄、不同性别以及不同部位鸡肉的脂质组成存在显著差异。本研究采用 LC/MS 脂质组学方法，分析了不同年龄、不同性别以及不同部位泰和乌鸡肌肉中的脂质组成。利用正交偏最小二乘判别分析和单因素方差分析对数据进行处理，共检测到 1127 种脂质。其中，有 88、11 和 1 种脂质分别在不同年龄、性别和部位组间的投影值>1 和 P 值<0.05 均具有显著差异。结果表明，年龄、性别和部位 3 个因素对泰和乌鸡肌肉脂质组成的影响顺序为年龄>性别>部位。脂质组成的差异有助于更好地理解泰和乌鸡的药用特性。本研究采用相同策略对泰和乌鸡和杂交乌鸡的脂质组成进行了比较，结果表明两组能够有效区分。共鉴定出 47 种脂质化合物可作为泰和乌鸡的潜在鉴定标志物。该研究成功地将脂质组学应用于食品原料的脂质分析。
