Ji'an Key Laboratory of Genetics, Breeding and Reproduction in Taihe Silky Fowl, College of Life Sciences, Jinggangshan University, Ji'an, Jiangxi, China.
Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province, College of Biological and Food Engineering, Fuyang Normal University, Fuyang, Anhui, China.
PLoS One. 2024 Mar 13;19(3):e0299385. doi: 10.1371/journal.pone.0299385. eCollection 2024.
The Taihe Black-Bone silky fowl chicken (BB-sfc) is a renowned dietary and medicinal chicken globally recognized for its high nutritional and medicinal value. Compared to the local Black-Bone black-feathered chicken (BB-bfc), the Taihe silky fowl chicken has higher levels of amino acids, trace elements, and unsaturated fatty acids in their muscles, which offer anti-aging, anti-cancer, and immune enhancing benefits. Despite this, the unique nutritional components, genes, and proteins in Taihe silky fowl chicken muscles are largely unknown. Therefore, we performed a comprehensive transcriptome and proteome analysis of muscle development between BB-sfc and BB-bfc chickens using RNA-Seq and TMT-based quantitative proteomics methods. RNA-Seq analysis identified 286 up-regulated genes and 190 down-regulated genes in BB-sfc chickens, with oxidoreductase activity and electron transfer activity enriched in up-regulated genes, and phospholipid homeostasis and cholesterol transporter activity enriched in down-regulated genes. Proteome analysis revealed 186 significantly increased and 287 significantly decreased proteins in Taihe BB-sfc chicken muscles, primarily affecting mitochondrial function and oxidative phosphorylation, crucial for enhancing muscle antioxidant capacity. Integrated transcriptome and proteome analysis identified 6 overlapped up-regulated genes and 8 overlapped down-regulated genes in Taihe silky fowl chicken, related to improved muscle antioxidant status. Taken together, this research provides a comprehensive database of gene expression and protein information in Taihe Black-Bone silky fowl chicken muscles, aiding in fully exploring their unique economic value in the future.
泰和乌骨鸡（BB-sfc）是一种著名的食用和药用鸡，因其具有高营养价值和药用价值而在全球范围内受到认可。与当地的黑羽乌鸡（BB-bfc）相比，泰和丝羽鸡的肌肉中氨基酸、微量元素和不饱和脂肪酸含量更高，具有抗衰老、抗癌和增强免疫力的功效。尽管如此，泰和丝羽鸡肌肉中的独特营养成分、基因和蛋白质在很大程度上仍未被了解。因此，我们使用 RNA-Seq 和 TMT 定量蛋白质组学方法对泰和丝羽鸡和黑羽乌鸡的肌肉发育进行了全面的转录组和蛋白质组分析。RNA-Seq 分析发现，泰和丝羽鸡中有 286 个上调基因和 190 个下调基因，其中上调基因富集了氧化还原酶活性和电子传递活性，下调基因富集了磷脂稳态和胆固醇转运蛋白活性。蛋白质组分析显示，泰和丝羽鸡的肌肉中有 186 个显著增加的蛋白质和 287 个显著减少的蛋白质，主要影响线粒体功能和氧化磷酸化，这对增强肌肉抗氧化能力至关重要。转录组和蛋白质组综合分析鉴定出 6 个上调基因和 8 个下调基因在泰和丝羽鸡中重叠，与改善肌肉抗氧化状态有关。综上所述，本研究为泰和乌骨鸡肌肉的基因表达和蛋白质信息提供了一个全面的数据库，有助于未来充分挖掘其独特的经济价值。
