Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China.
Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, PR China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, PR China.
Chemosphere. 2018 Sep;207:337-346. doi: 10.1016/j.chemosphere.2018.05.098. Epub 2018 May 19.
Fine particulate matter (PM) has been listed as an important environmental risk factor for human health. However, the systemic biological effects on metabolic responses induced by PM and its components were poorly understood. This study was aimed to evaluate the toxicity of different components of PM at molecular level via metabolomics approach. In the present study, we adopted a H NMR-based metabolomics approach to evaluate metabolic profiles in mice after acute exposure to Total-PM, water soluble components of PM (WS-PM) and water insoluble components of PM (WIS-PM). First, we characterized the morphological features and chemical composition of PM. Then, the metabolites changes of serum and urine in mice were systematically analyzed using 800 MHz H NMR techniques in combination with multivariate statistical analysis. Total-PM exposure affected metabolites mainly involved in amino acid metabolism, protein biosynthesis, energy metabolism and metabolism of cofactors and vitamins. WS-PM exposure influenced lipid metabolism and carbohydrate metabolism. WIS-PM exposure mainly perturbed amino acid metabolism and energy metabolism. The results suggested that acute exposure to the Total-PM, WS-PM and WIS-PM in mice exhibited marked systemic metabolic changes. In addition, the insoluble fraction of PM contributed greatly to the toxicity of PM
细颗粒物（PM）已被列为影响人类健康的重要环境风险因素。然而，人们对 PM 及其成分引起的代谢反应的系统生物学效应仍知之甚少。本研究旨在通过代谢组学方法评估 PM 不同成分的分子水平毒性。在本研究中，我们采用基于 1 H NMR 的代谢组学方法，评估了急性暴露于总 PM、PM 的水溶性成分（WS-PM）和不溶性成分（WIS-PM）后小鼠的代谢谱。首先，我们对 PM 的形态特征和化学成分进行了表征。然后，使用 800 MHz 1 H NMR 技术结合多变量统计分析，系统分析了血清和尿液中代谢物的变化。总 PM 暴露影响的代谢物主要涉及氨基酸代谢、蛋白质生物合成、能量代谢以及辅因子和维生素代谢。WS-PM 暴露影响脂质代谢和碳水化合物代谢。WIS-PM 暴露主要干扰氨基酸代谢和能量代谢。结果表明，急性暴露于总 PM、WS-PM 和 WIS-PM 的小鼠表现出明显的系统性代谢变化。此外，PM 的不溶性部分对 PM 的毒性有很大贡献。
